Detailed information of TA system TA225036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1105833..1106517
• Replicon: chromosome
• Accession: NZ_CP087183
• Organism: Pseudomonas putida strain B21-029

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: LOY24_RS04940
• Protein ID: WP_258655404.1
• Coordinates: 1106146..1106517 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: LOY24_RS04935
• Protein ID: WP_258655403.1
• Coordinates: 1105833..1106153 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY24_RS04910 (LOY24_04910)	1102018..1102419	+	402	WP_258655399.1	FagA protein	-
LOY24_RS04915 (LOY24_04915)	1102412..1103788	+	1377	WP_043196908.1	class II fumarate hydratase	-
LOY24_RS04920 (LOY24_04920)	1103817..1104278	+	462	WP_258655400.1	DUF2753 domain-containing protein	-
LOY24_RS04925 (LOY24_04925)	1104280..1104891	+	612	WP_258655401.1	superoxide dismutase	-
LOY24_RS04930 (LOY24_04930)	1104903..1105796	+	894	WP_258655402.1	ZIP family metal transporter	-
LOY24_RS04935 (LOY24_04935)	1105833..1106153	-	321	WP_258655403.1	helix-turn-helix transcriptional regulator	Antitoxin
LOY24_RS04940 (LOY24_04940)	1106146..1106517	-	372	WP_258655404.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY24_RS04945 (LOY24_04945)	1106606..1106878	-	273	WP_003255140.1	HPr family phosphocarrier protein	-
LOY24_RS04950 (LOY24_04950)	1106898..1107752	-	855	WP_016501338.1	RNase adapter RapZ	-
LOY24_RS04955 (LOY24_04955)	1107755..1108219	-	465	WP_016501337.1	PTS IIA-like nitrogen regulatory protein PtsN	-
LOY24_RS04960 (LOY24_04960)	1108232..1108540	-	309	WP_016501336.1	ribosome-associated translation inhibitor RaiA	-
LOY24_RS04965 (LOY24_04965)	1108620..1110113	-	1494	WP_258655405.1	RNA polymerase factor sigma-54	-
LOY24_RS04970 (LOY24_04970)	1110289..1111014	-	726	WP_016501334.1	LPS export ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-111)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-90)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13693.61 Da
• Isoelectric Point: 9.4692

>T225036 WP_258655404.1 NZ_CP087183:c1106517-1106146 [Pseudomonas putida]
MDALKRIRALYWVGSSKKDLQALPEDVQDIFGFALHLAQEGGKHQQTKCLKGFSGAGVLEIMEDHNRNTYRAVYTVSFGS
AVYVLHCFQKKSVSGIKTSSHDIALIRSRLKLAEAHSRGETND

Nucleotide

• Length: 372 bp

>T225036 NZ_CP120847:c4762993-4762460 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11545.59 Da
• Isoelectric Point: 10.2100

>AT225036 WP_258655403.1 NZ_CP087183:c1106153-1105833 [Pseudomonas putida]
MTEIEAGSGNVYADLNGADANEMLVKAQLASKICSIIKARHLTQLQAAAVLGLPQPKVSEMMRGKFRGISETKMIDCLAR
LGRDIQIVVKPAPRSRREGRVEVIIT

Nucleotide

• Length: 321 bp

>AT225036 NZ_CP120847:c4763270-4763004 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA