Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/-
Location 6949888..6950495 Replicon chromosome
Accession NZ_CP087182
Organism Pseudomonas protegens strain B21-030

Toxin (Protein)


Gene name PfiT Uniprot ID A0A7U9LCE3
Locus tag LOY23_RS31915 Protein ID WP_025131511.1
Coordinates 6949888..6950232 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A7Y1CEW2
Locus tag LOY23_RS31920 Protein ID WP_025131512.1
Coordinates 6950247..6950495 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY23_RS31900 (LOY23_31855) 6945973..6946464 - 492 WP_011064291.1 5-(carboxyamino)imidazole ribonucleotide mutase -
LOY23_RS31905 (LOY23_31860) 6946989..6947903 - 915 WP_011064292.1 LysR substrate-binding domain-containing protein -
LOY23_RS31910 (LOY23_31865) 6948085..6949509 + 1425 WP_041120431.1 aspartate ammonia-lyase -
LOY23_RS31915 (LOY23_31870) 6949888..6950232 - 345 WP_025131511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOY23_RS31920 (LOY23_31875) 6950247..6950495 - 249 WP_025131512.1 prevent-host-death protein Antitoxin
LOY23_RS31925 (LOY23_31880) 6951104..6951460 + 357 WP_123431125.1 DUF3077 domain-containing protein -
LOY23_RS31930 (LOY23_31885) 6951663..6952388 + 726 WP_072443427.1 hypothetical protein -
LOY23_RS31935 (LOY23_31890) 6952482..6953129 + 648 WP_065365015.1 YcxB family protein -
LOY23_RS31940 (LOY23_31895) 6953177..6954196 + 1020 WP_174395856.1 beta-ketoacyl-ACP synthase 3 -
LOY23_RS31945 (LOY23_31900) 6954196..6955203 + 1008 WP_258718076.1 NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12896.62 Da        Isoelectric Point: 4.2986

>T225035 WP_025131511.1 NZ_CP087182:c6950232-6949888 [Pseudomonas protegens]
MPTLTIRYTETAQQSIEDQIVHLADYSDLKSAFERLEAVIDALQDKLLSAPLGYPVSPQASDLGILHYRELNTDGYRVFY
EIVDADQAIVIGLVLRDKQSVEQALIRYCLLHTD

Download         Length: 345 bp

>T225035 NZ_CP120847:4128548-4128766 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9410.89 Da        Isoelectric Point: 9.8184

>AT225035 WP_025131512.1 NZ_CP087182:c6950495-6950247 [Pseudomonas protegens]
MRVETVSFLKRYASDLPLEEPMVVTQNGVPVYVVESYVERKRRDEAVALVKLLAVSSREYAQGKHCSTDELKARLSKRFA
HK

Download         Length: 249 bp

>AT225035 NZ_CP120847:4128147-4128521 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LCE3

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y1CEW2

References