Detailed information of TA system TA225032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3059742..3060990
• Replicon: chromosome
• Accession: NZ_CP087182
• Organism: Pseudomonas protegens strain B21-030

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOY23_RS14205
• Protein ID: WP_210482173.1
• Coordinates: 3060418..3060990 (+)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOY23_RS14200
• Protein ID: WP_258718452.1
• Coordinates: 3059742..3060299 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY23_RS14170 (LOY23_14155)	3054946..3055596	+	651	WP_103291076.1	DUF2239 family protein	-
LOY23_RS14175 (LOY23_14160)	3055684..3056304	+	621	WP_011060979.1	LysE family translocator	-
LOY23_RS14180 (LOY23_14165)	3056350..3056937	-	588	WP_011060980.1	AidA/PixA family protein	-
LOY23_RS14185 (LOY23_14170)	3057397..3058584	+	1188	WP_258718450.1	PrpF domain-containing protein	-
LOY23_RS14190 (LOY23_14175)	3058763..3059209	-	447	WP_258718451.1	hypothetical protein	-
LOY23_RS14195 (LOY23_14180)	3059211..3059552	-	342	WP_053158065.1	low molecular weight protein tyrosine phosphatase family protein	-
LOY23_RS14200 (LOY23_14185)	3059742..3060299	+	558	WP_258718452.1	helix-turn-helix transcriptional regulator	Antitoxin
LOY23_RS14205 (LOY23_14190)	3060418..3060990	+	573	WP_210482173.1	HD domain-containing protein	Toxin
LOY23_RS14210 (LOY23_14195)	3061245..3063425	+	2181	WP_258718453.1	catalase/peroxidase HPI	-
LOY23_RS14215 (LOY23_14200)	3063636..3064094	+	459	WP_015635386.1	hypothetical protein	-
LOY23_RS14220 (LOY23_14205)	3064286..3064714	-	429	WP_053158074.1	hypothetical protein	-
LOY23_RS14225 (LOY23_14210)	3064949..3065863	+	915	WP_258718454.1	SGNH/GDSL hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (8-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-48)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21385.03 Da
• Isoelectric Point: 4.9459

>T225032 WP_210482173.1 NZ_CP087182:3060418-3060990 [Pseudomonas protegens]
MNSELLSGRLDFLREAEKLKDVLRSAHTSSGRQESTAEHSWRLCLMALLFEDQLGDLDLLRVLKLCIVHDLGEAIHGDIP
AVVQAAHPDKSQQERADLQLLARALDAPAREHLLELWDEYEGAASAEAQTVKALDKLETLLQHTQGQNPAGFDYRFNLGY
GRRYTDASPLFQALRQQIDIDTQRRLDAQG

Nucleotide

• Length: 573 bp

>T225032 NZ_CP120847:2040494-2040596 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20469.34 Da
• Isoelectric Point: 7.0311

>AT225032 WP_258718452.1 NZ_CP087182:3059742-3060299 [Pseudomonas protegens]
MSVAPFTSPHLGLALRRWRLLHRVKQQHAAELFGVAQSSISRWENGRQTMEPAERARLEQLLAANLSAAADQALARLVND
SPRAVHLVCDLTHRLLACSATRAGQFGVPLSELLGQSLWGFCTEEITRKEAALDDLGWREVLEPPSLEFFSGHNDSAIVP
IAPSQCRWTRLNLSDGNAVRLVETL

Nucleotide

• Length: 558 bp

>AT225032 NZ_CP120847:c2040603-2040458 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT