Detailed information of TA system TA225028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 298600..299213
• Replicon: chromosome
• Accession: NZ_CP087182
• Organism: Pseudomonas protegens strain B21-030

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9LBG9
• Locus tag: LOY23_RS01340
• Protein ID: WP_041115175.1
• Coordinates: 298600..298782 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY23_RS01345
• Protein ID: WP_041120520.1
• Coordinates: 298812..299213 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY23_RS01320 (LOY23_01320)	294338..296299	+	1962	WP_161466434.1	choline transporter BetT	-
LOY23_RS01325 (LOY23_01325)	296309..296566	-	258	WP_015633747.1	hypothetical protein	-
LOY23_RS01330 (LOY23_01330)	296619..297359	-	741	WP_015633748.1	SDR family oxidoreductase	-
LOY23_RS01335 (LOY23_01335)	297492..298382	+	891	WP_011058633.1	LysR family transcriptional regulator	-
LOY23_RS01340 (LOY23_01340)	298600..298782	+	183	WP_041115175.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY23_RS01345 (LOY23_01345)	298812..299213	+	402	WP_041120520.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY23_RS01350 (LOY23_01350)	299226..300272	-	1047	WP_077933197.1	LacI family DNA-binding transcriptional regulator	-
LOY23_RS01355 (LOY23_01355)	300478..302736	+	2259	WP_077933198.1	TonB-dependent receptor	-
LOY23_RS01360 (LOY23_01360)	302880..304208	+	1329	WP_077933199.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6866.97 Da
• Isoelectric Point: 10.8547

>T225028 WP_041115175.1 NZ_CP087182:298600-298782 [Pseudomonas protegens]
MRSREMISRIEADGWYLVAVKGSHHQYKHPFKPGRVTIRHPDTDLPKGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T225028 NZ_CP120846:c1389657-1388911 [Bacillus atrophaeus]
ATGCTTTTATTCTTTCAGATTATGGTTTGGTGCATAATGGCCGCATTAGGCCTATACGTATATGCGGTGTGGCGGTTTGA
AGCAAAGGTGAAAGAAAAAATGTTTGCGATCCGGAAAACGTGGTATTTGATCTTTGTGATCGGCGCCATGGTATACTGGA
CGTATGACCCCTCTTCCCTTTTCAGGGAATGGACGAATTATTTGATTGTCGCTGTCAGCTTTGCTTTGATTGACGCCTTT
ATCTTTTTGAGTGCTTACGTAAAGAAACTTGCCGGCAATGAATTAGAAACCGACACACGGGAAATCCTTGAAGAAAACAA
TGAAATGCTCCACGTTTATCTTGATAAATTAAAAACGTACCAATACCTATTGAAAAATGAACCGATCCATGTTTATTATG
GAAGTATAGAAGCATACGCCGAGGGCATCGATAAGCTGCTCAAAACCTATGCCGACAAAATGAACTTAACGGCTTCGCTG
TGTGAATATTCGACACAGCTTGAAAAAGACCGCTTAACCGAGCATATGGATGATGCATTTGAGGTGCAGAGCCGGCTGGA
TCGAAAAGATGTATACTATGATCAGCACGGGAAGCTGGCACTCATCCCTTTTACAGTCCAGACACGCAGCTACGTCATCA
AGCTGGCGTCAGACAGCATTGTAACAGAATTTGATTATTTGCTTTTCACGTCATTAACTAGCATTTACGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGACGGATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14723.45 Da
• Isoelectric Point: 4.5430

>AT225028 WP_041120520.1 NZ_CP087182:298812-299213 [Pseudomonas protegens]
MKFPVVLHKDADSEYGVTVPDVPGCHSAGPTVSLAFENIKEALSLHYEGLVADGEPLPQVHEIDDHLDNPDYAGGVWGVV
EFDVTPYFGKSVRFNATLPEQLLERIDQRVERDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT225028 NZ_CP120846:c1388911-1388741 [Bacillus atrophaeus]
ATGGAACGTGCTTTTCAAAACGATCATTCGCCCCGCGCGGCTAAACCGTTTAAGATATTAAAAAAGAGATCAACCACCAG
CGTCGCAAGCTATCAGGTCAGCCCCCACACGGCAAGAATTTTCAAAGAAAATGAGCGGCTGATTGACGAGTATAAAAGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A7U9LBG9