Detailed information of TA system TA225027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5109654..5110285
• Replicon: chromosome
• Accession: NZ_CP087181
• Organism: Pseudomonas sp. B21-031

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY53_RS22855
• Protein ID: WP_099430453.1
• Coordinates: 5110058..5110285 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY53_RS22850
• Protein ID: WP_099430452.1
• Coordinates: 5109654..5110061 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY53_RS22830 (LOY53_22820)	5106301..5107623	+	1323	WP_258703313.1	phosphatase PAP2/dual specificity phosphatase family protein	-
LOY53_RS22835 (LOY53_22825)	5107620..5108051	+	432	WP_252825874.1	hypothetical protein	-
LOY53_RS22840 (LOY53_22830)	5108062..5108685	+	624	WP_027595452.1	lysophospholipid acyltransferase family protein	-
LOY53_RS22845 (LOY53_22835)	5108687..5109619	+	933	WP_027595453.1	phosphatidate cytidylyltransferase	-
LOY53_RS22850 (LOY53_22840)	5109654..5110061	-	408	WP_099430452.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY53_RS22855 (LOY53_22845)	5110058..5110285	-	228	WP_099430453.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY53_RS22860 (LOY53_22850)	5110561..5110905	+	345	WP_003251579.1	cupin domain-containing protein	-
LOY53_RS22865 (LOY53_22855)	5111240..5112127	-	888	WP_027595454.1	3-hydroxyisobutyrate dehydrogenase	-
LOY53_RS22870 (LOY53_22860)	5112138..5113664	-	1527	WP_085273919.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
LOY53_RS22875 (LOY53_22865)	5113797..5114747	+	951	WP_258703314.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5107994..5131555	23561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8315.30 Da
• Isoelectric Point: 10.5627

>T225027 WP_099430453.1 NZ_CP087181:c5110285-5110058 [Pseudomonas sp. B21-031]
MRSREVIQLIEADGWYEVEVKGSHHQFRHPTKRGRVTVPHPKSDLPKGTVHSILKQAGLKQPNGSNSSSTSGDTQ

Nucleotide

• Length: 228 bp

>T225027 NZ_CP120846:533219-533569 [Bacillus atrophaeus]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGAGTGCGCCCGGT
TTTGGTGATTCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATTTTGCTGGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATTAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14672.49 Da
• Isoelectric Point: 4.7169

>AT225027 WP_099430452.1 NZ_CP087181:c5110061-5109654 [Pseudomonas sp. B21-031]
MKFPVVLHKDADSDYGVTVPDVPGCFSAGASVSQALENVQEALALHFEGLVADNEALPQAQEIDVHVANPDYQGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELAIAH

Nucleotide

• Length: 408 bp

>AT225027 NZ_CP120846:532933-533214 [Bacillus atrophaeus]
TTGTCTGAATCCAGCGCAACAACCGAAATGATGATTAGCTTGCCGGAAGCACTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTAATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGCACAACTCGTCACAACC
GTGATTTGATGAGACGCGGATATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAAGCTCATTTTGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA