Detailed information of TA system TA225026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-DUF971
• Location: 4389131..4389719
• Replicon: chromosome
• Accession: NZ_CP087181
• Organism: Pseudomonas sp. B21-031

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LOY53_RS19595
• Protein ID: WP_252825351.1
• Coordinates: 4389131..4389433 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LOY53_RS19600
• Protein ID: WP_027593133.1
• Coordinates: 4389426..4389719 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY53_RS19570	4385006..4385134	-	129	WP_256214945.1	PA1414 family protein	-
LOY53_RS19575 (LOY53_19570)	4385259..4386152	-	894	WP_027593129.1	LysR family transcriptional regulator	-
LOY53_RS19580 (LOY53_19575)	4386259..4387440	+	1182	WP_258703156.1	MFS transporter	-
LOY53_RS19585 (LOY53_19580)	4387574..4388500	-	927	WP_099430143.1	DMT family transporter	-
LOY53_RS19590 (LOY53_19585)	4388606..4389001	-	396	WP_027593132.1	hypothetical protein	-
LOY53_RS19595 (LOY53_19590)	4389131..4389433	+	303	WP_252825351.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY53_RS19600 (LOY53_19595)	4389426..4389719	+	294	WP_027593133.1	putative addiction module antidote protein	Antitoxin
LOY53_RS19605 (LOY53_19600)	4389832..4390104	+	273	WP_099455003.1	hypothetical protein	-
LOY53_RS19610 (LOY53_19605)	4390161..4391522	-	1362	WP_027593135.1	DEAD/DEAH box helicase	-
LOY53_RS19615 (LOY53_19610)	4391627..4392913	+	1287	WP_099455004.1	mechanosensitive ion channel family protein	-
LOY53_RS19620 (LOY53_19615)	4393028..4393963	-	936	WP_252825353.1	AEC family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4381325..4400391	19066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-87)

Antitoxin

• Uncharacterized protein HI1420 (8-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11432.12 Da
• Isoelectric Point: 10.5383

>T225026 WP_252825351.1 NZ_CP087181:4389131-4389433 [Pseudomonas sp. B21-031]
MKKIESSSFRHWVHGLRDEMARIRIIARINRLMEGLSGDVSAVGHGVSELRIHHGPGYRVYFHQAGDTLVILLCGGDKSS
QRRDIKAAHEILRSWRMQND

Nucleotide

• Length: 303 bp

>T225026 NZ_CP120838:10762-11067 [Salmonella enterica subsp. enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10517.96 Da
• Isoelectric Point: 5.5740

>AT225026 WP_027593133.1 NZ_CP087181:4389426-4389719 [Pseudomonas sp. B21-031]
MTETFFEFDAADYLTTPQAIAQFMSDALETGDASYVAKAVGVVARAKGMSDLAKETGLSREQLYRSFSEHGNPTLKSFLA
VMKALGIDMTAKPHLAH

Nucleotide

• Length: 294 bp

>AT225026 NZ_CP120838:10542-10760 [Salmonella enterica subsp. enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA