Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/upstrm_HI1419-dnstrm_HI1420
Location 1018054..1018649 Replicon chromosome
Accession NZ_CP087178
Organism Pseudomonas sp. B21-036

Toxin (Protein)


Gene name relE Uniprot ID A0A1Y3KHK4
Locus tag LOY33_RS04445 Protein ID WP_043864446.1
Coordinates 1018054..1018347 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1Y3KHQ4
Locus tag LOY33_RS04450 Protein ID WP_043864447.1
Coordinates 1018350..1018649 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY33_RS04425 (LOY33_04420) 1014067..1015212 - 1146 WP_258661874.1 FMNH2-dependent alkanesulfonate monooxygenase -
LOY33_RS04430 (LOY33_04425) 1015209..1015787 - 579 WP_043864443.1 FMN reductase -
LOY33_RS04435 (LOY33_04430) 1016026..1016808 - 783 WP_258661876.1 helix-turn-helix transcriptional regulator -
LOY33_RS04440 (LOY33_04435) 1016902..1017810 + 909 WP_258661879.1 EamA family transporter -
LOY33_RS04445 (LOY33_04440) 1018054..1018347 + 294 WP_043864446.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOY33_RS04450 (LOY33_04445) 1018350..1018649 + 300 WP_043864447.1 putative addiction module antidote protein Antitoxin
LOY33_RS04455 (LOY33_04450) 1018869..1019771 - 903 WP_043864448.1 transporter substrate-binding domain-containing protein -
LOY33_RS04460 (LOY33_04455) 1019802..1020152 - 351 WP_258661884.1 RidA family protein -
LOY33_RS04465 (LOY33_04460) 1020178..1021464 - 1287 WP_258662861.1 D-amino acid dehydrogenase -
LOY33_RS04470 (LOY33_04465) 1021527..1022432 + 906 WP_088515308.1 LysR substrate-binding domain-containing protein -
LOY33_RS04475 (LOY33_04470) 1022888..1023448 + 561 WP_143516664.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-96)

Antitoxin

Uncharacterized protein HI1420

(5-95)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10480.14 Da        Isoelectric Point: 10.6099

>T225020 WP_043864446.1 NZ_CP087178:1018054-1018347 [Pseudomonas sp. B21-036]
VIYSIEQTLAFSGWLLALRDVRARIAIGRRIERALAGNLGDTKAVGDGVSEMRIDSGPGYRVYFTVRSGVAILLLTGGDK
SSQAADIRMAKRLAKEV

Download         Length: 294 bp

>T225020 NZ_CP120837:c3987523-3987236 [Salmonella enterica subsp. enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10599.26 Da        Isoelectric Point: 5.6850

>AT225020 WP_043864447.1 NZ_CP087178:1018350-1018649 [Pseudomonas sp. B21-036]
MSVTLIPFDMAALLDSDEAISEYLSQVLAEGDSDELLRAIGYVAKARGMSLVAKETGLGRESLYKAFAPGAKPRFDTVLK
VMHALGIELCARSHRGASV

Download         Length: 300 bp

>AT225020 NZ_CP120837:c3987249-3986947 [Salmonella enterica subsp. enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y3KHK4

Antitoxin

Source ID Structure
AlphaFold DB A0A1Y3KHQ4

References