Detailed information of TA system TA022502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 5286361..5286954
• Replicon: chromosome
• Accession: NC_009925
• Organism: Acaryochloris marina MBIC11017

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B0C9N5
• Locus tag: AM1_RS23645
• Protein ID: WP_012165444.1
• Coordinates: 5286361..5286657 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AM1_RS23650
• Protein ID: WP_041661024.1
• Coordinates: 5286661..5286954 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM1_RS23630	5281565..5284024	+	2460	WP_012165440.1	DUF11 domain-containing protein	-
AM1_RS23635	5284073..5285284	-	1212	WP_012165441.1	saccharopine dehydrogenase NADP-binding domain-containing protein	-
AM1_RS23640	5285429..5285719	+	291	WP_012165442.1	DUF2288 domain-containing protein	-
AM1_RS42290	5285829..5286116	-	288	WP_187152901.1	hypothetical protein	-
AM1_RS23645	5286361..5286657	+	297	WP_012165444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM1_RS23650	5286661..5286954	+	294	WP_041661024.1	putative addiction module antidote protein	Antitoxin
AM1_RS23655	5287154..5287585	+	432	WP_012165446.1	dUTP diphosphatase	-
AM1_RS23660	5287673..5287945	-	273	WP_012165447.1	hypothetical protein	-
AM1_RS23665	5288252..5290729	-	2478	WP_041660301.1	ATP-dependent DNA helicase RecG	-
AM1_RS23670	5290764..5291294	-	531	WP_012165449.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11133.69 Da
• Isoelectric Point: 9.9108

>T22502 WP_012165444.1 NC_009925:5286361-5286657 [Acaryochloris marina MBIC11017]
MIEVRQTDIFAKWFNNLRDRKAKARVQARIDRIEIGNFGDVAPVGEGVSELRIHYGPGYRVYFVQRDSVVVILLSGGDKS
SQNADIKRAKEIAKQLED

Nucleotide

• Length: 297 bp

>T22502 NC_009925:5286361-5286657 [Acaryochloris marina MBIC11017]
ATGATTGAGGTTCGTCAAACGGACATTTTTGCCAAGTGGTTCAATAATCTGCGAGATCGCAAAGCTAAAGCTCGTGTACA
AGCACGTATTGACCGTATCGAGATTGGTAACTTTGGCGATGTTGCCCCTGTGGGAGAAGGCGTTAGCGAACTCCGCATTC
ACTATGGTCCGGGGTACAGGGTTTACTTTGTACAGCGCGATTCAGTTGTTGTCATCTTGCTCTCTGGTGGTGATAAAAGC
TCCCAAAATGCTGACATTAAAAGGGCTAAGGAAATTGCTAAACAGTTGGAGGATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10536.03 Da
• Isoelectric Point: 4.7627

>AT22502 WP_041661024.1 NC_009925:5286661-5286954 [Acaryochloris marina MBIC11017]
MPISTTYWDSAAHLKTDEDIQLYLEACIEEAEDDPALIIHALGVVARAKNMSQLSRETGLSREGLYKALSVDGNPTFATI
AKVTKALGFKLTIQPTA

Nucleotide

• Length: 294 bp

>AT22502 NC_009925:5286661-5286954 [Acaryochloris marina MBIC11017]
ATGCCCATAAGCACAACCTATTGGGATTCTGCTGCACATCTCAAAACAGACGAAGATATTCAGCTCTACCTAGAAGCCTG
CATTGAAGAAGCAGAAGATGATCCTGCTTTAATTATTCATGCCCTAGGAGTTGTCGCCAGGGCGAAAAACATGAGTCAAC
TCTCCCGAGAAACAGGTTTGAGTAGAGAGGGCCTATATAAAGCGCTGTCAGTTGATGGCAACCCCACGTTTGCGACTATC
GCTAAAGTCACTAAAGCACTCGGATTCAAGCTAACCATTCAGCCAACGGCTTAG

Structures

Toxin

• AlphaFold DB: B0C9N5