Detailed information of TA system TA225018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 5978877..5980501
• Replicon: chromosome
• Accession: NZ_CP087177
• Organism: Pseudomonas moraviensis strain B21-039

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LOY57_RS26455
• Protein ID: WP_258639771.1
• Coordinates: 5979152..5980501 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V8R249
• Locus tag: LOY57_RS26450
• Protein ID: WP_024014849.1
• Coordinates: 5978877..5979155 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY57_RS26420 (LOY57_26375)	5974308..5974931	+	624	WP_116657239.1	FMN-binding negative transcriptional regulator	-
LOY57_RS26425 (LOY57_26380)	5974942..5975388	+	447	WP_064365159.1	GNAT family N-acetyltransferase	-
LOY57_RS26430 (LOY57_26385)	5975401..5976072	+	672	WP_065616395.1	GNAT family protein	-
LOY57_RS26435 (LOY57_26390)	5976194..5977096	-	903	WP_065616396.1	homocysteine S-methyltransferase family protein	-
LOY57_RS26440 (LOY57_26395)	5977210..5978028	+	819	WP_258639769.1	ABC transporter substrate-binding protein	-
LOY57_RS26445 (LOY57_26400)	5978025..5978861	+	837	WP_064365163.1	amino acid ABC transporter permease	-
LOY57_RS26450 (LOY57_26405)	5978877..5979155	-	279	WP_024014849.1	helix-turn-helix transcriptional regulator	Antitoxin
LOY57_RS26455 (LOY57_26410)	5979152..5980501	-	1350	WP_258639771.1	HipA domain-containing protein	Toxin
LOY57_RS26460 (LOY57_26415)	5980871..5983066	+	2196	WP_258639773.1	autotransporter outer membrane beta-barrel domain-containing protein	-
LOY57_RS26465 (LOY57_26420)	5983261..5985069	-	1809	WP_116657233.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49837.42 Da
• Isoelectric Point: 5.2671

>T225018 WP_258639771.1 NZ_CP087177:c5980501-5979152 [Pseudomonas moraviensis]
MYNLTLQVFTAGQWHDAMTLDFDDPEKGFESRCSFGYEAGYLVENIELIGSPFSKAVSALYPLDWEGRRAKTPAFVHDIA
PAGAAKRFLLARLGQERPAEISADLFLLGRRTPAPIGNMRIKESAEAVDERKPIGFKREEVISRDNRFLEYAYELGAAIG
GATGAGGEAPKLLLAENSAGLLYPDAVLDDEEVRQHWFVKFARNRGGQTDQDILRSEFHYYKALQTLGIETVAAEGLALE
EANKPSLWMRRFDRQVEDGAVSRFAVESIYSLAEVTTPGSAMNHLDVIRMLAGLWRKAGQASQIPGLVADYLRRDLINKI
LGNSDNHGRNTAIIRQENAFRLAPIYDLAPMVMDDEGVTRTTKWTKELERAGDVDWRGVCRVLADSADPHDSLAGLDASA
LFEGLRADARRLAALPDILAASGLPARTMNHPSVHLKNLEQRLKEWDLQ

Nucleotide

• Length: 1350 bp

>T225018 NZ_CP120837:3411827-3412045 [Salmonella enterica subsp. enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10318.09 Da
• Isoelectric Point: 10.7364

>AT225018 WP_024014849.1 NZ_CP087177:c5979155-5978877 [Pseudomonas moraviensis]
MSLTVLERTALIESIQQSLAEGTLEIGDAVRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGM
KMGVVRIRRDTV

Nucleotide

• Length: 279 bp

>AT225018 NZ_CP120837:3411425-3411799 [Salmonella enterica subsp. enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1N7C5J7