Detailed information of TA system TA225013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1768192..1768808
• Replicon: chromosome
• Accession: NZ_CP087177
• Organism: Pseudomonas moraviensis strain B21-039

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY57_RS07740
• Protein ID: WP_258640854.1
• Coordinates: 1768192..1768404 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A423NEP3
• Locus tag: LOY57_RS07745
• Protein ID: WP_042607564.1
• Coordinates: 1768404..1768808 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY57_RS07710 (LOY57_07690)	1764294..1764830	+	537	WP_064365457.1	DsbE family thiol:disulfide interchange protein	-
LOY57_RS07715 (LOY57_07695)	1764827..1765297	+	471	WP_064365456.1	cytochrome c-type biogenesis protein CcmH	-
LOY57_RS07720 (LOY57_07700)	1765294..1766493	+	1200	WP_252881526.1	c-type cytochrome biogenesis protein CcmI	-
LOY57_RS07725 (LOY57_07705)	1766519..1766923	+	405	WP_064365455.1	hypothetical protein	-
LOY57_RS07730 (LOY57_07710)	1766968..1767543	-	576	WP_258640853.1	PIN domain-containing protein	-
LOY57_RS07735 (LOY57_07715)	1767540..1768007	-	468	WP_042607562.1	helix-turn-helix domain-containing protein	-
LOY57_RS07740 (LOY57_07720)	1768192..1768404	+	213	WP_258640854.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY57_RS07745 (LOY57_07725)	1768404..1768808	+	405	WP_042607564.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY57_RS07750 (LOY57_07730)	1768921..1770120	-	1200	WP_258640855.1	MFS transporter	-
LOY57_RS07755 (LOY57_07735)	1770529..1771527	-	999	WP_258640856.1	sulfate ABC transporter substrate-binding protein	-
LOY57_RS07760 (LOY57_07740)	1771704..1772528	-	825	WP_064365449.1	ion transporter	-
LOY57_RS07765 (LOY57_07745)	1772550..1773464	-	915	WP_065615109.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7937.04 Da
• Isoelectric Point: 10.5973

>T225013 WP_258640854.1 NZ_CP087177:1768192-1768404 [Pseudomonas moraviensis]
VQSRLLMKELEEAGWTLDRVTGSHHIFTHRYNPNTIAVPHPKKDLPLGTVTSIRRRAGLFSPPTRHEGDP

Nucleotide

• Length: 213 bp

>T225013 NZ_CP120837:825264-825677 [Salmonella enterica subsp. enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14693.67 Da
• Isoelectric Point: 4.5685

>AT225013 WP_042607564.1 NZ_CP087177:1768404-1768808 [Pseudomonas moraviensis]
MQYPICIEWGDENTAIGIQIPDIPGADTAGDSFEEAYNAAVEVAHLMLQEIAAAGQAIPMPTSAAAHRSHPDFAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT225013 NZ_CP120837:825017-825283 [Salmonella enterica subsp. enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A423NEP3