Detailed information of TA system TA225012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1766968..1768007
• Replicon: chromosome
• Accession: NZ_CP087177
• Organism: Pseudomonas moraviensis strain B21-039

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY57_RS07730
• Protein ID: WP_258640853.1
• Coordinates: 1766968..1767543 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A2U1R6T9
• Locus tag: LOY57_RS07735
• Protein ID: WP_042607562.1
• Coordinates: 1767540..1768007 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY57_RS07705 (LOY57_07685)	1762309..1764297	+	1989	WP_258640852.1	heme lyase CcmF/NrfE family subunit	-
LOY57_RS07710 (LOY57_07690)	1764294..1764830	+	537	WP_064365457.1	DsbE family thiol:disulfide interchange protein	-
LOY57_RS07715 (LOY57_07695)	1764827..1765297	+	471	WP_064365456.1	cytochrome c-type biogenesis protein CcmH	-
LOY57_RS07720 (LOY57_07700)	1765294..1766493	+	1200	WP_252881526.1	c-type cytochrome biogenesis protein CcmI	-
LOY57_RS07725 (LOY57_07705)	1766519..1766923	+	405	WP_064365455.1	hypothetical protein	-
LOY57_RS07730 (LOY57_07710)	1766968..1767543	-	576	WP_258640853.1	PIN domain-containing protein	Toxin
LOY57_RS07735 (LOY57_07715)	1767540..1768007	-	468	WP_042607562.1	helix-turn-helix domain-containing protein	Antitoxin
LOY57_RS07740 (LOY57_07720)	1768192..1768404	+	213	WP_258640854.1	type II toxin-antitoxin system HicA family toxin	-
LOY57_RS07745 (LOY57_07725)	1768404..1768808	+	405	WP_042607564.1	type II toxin-antitoxin system HicB family antitoxin	-
LOY57_RS07750 (LOY57_07730)	1768921..1770120	-	1200	WP_258640855.1	MFS transporter	-
LOY57_RS07755 (LOY57_07735)	1770529..1771527	-	999	WP_258640856.1	sulfate ABC transporter substrate-binding protein	-
LOY57_RS07760 (LOY57_07740)	1771704..1772528	-	825	WP_064365449.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21607.66 Da
• Isoelectric Point: 6.1364

>T225012 WP_258640853.1 NZ_CP087177:c1767543-1766968 [Pseudomonas moraviensis]
VRHSSFTAVYDACVLYPAPLRDFLMWLALSGLYRARWTQEIHHEWKRNLLKNRSDLTLEQLDRTSQLMNLAIPDACIDGY
EDLVSGLTLPDENDRHVLAAAIRCGADVIVTFNLKDFPSCHLAPYGIEAQHPDEFVENLFHLDSAAVVAAAQSQRRQLKT
PTVEVDKYLGFLQRQGLVESVKALGNYRAIL

Nucleotide

• Length: 576 bp

>T225012 NZ_CP120837:358940-359215 [Salmonella enterica subsp. enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17223.59 Da
• Isoelectric Point: 6.0020

>AT225012 WP_042607562.1 NZ_CP087177:c1768007-1767540 [Pseudomonas moraviensis]
MPTPIPSTTNLPVEREVKAAIEGQRTLTAFLATGFDTQRIQIFDEQNEAHSVELPTSALRLLSDILAALAEGNAVKVVPI
HAELTTQEAADLLNVSRPHMIKLLESGEISYHKTGKHRRVRFADLMEYKTRRDTASEQAMTLLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT225012 NZ_CP120837:358677-358937 [Salmonella enterica subsp. enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U1R6T9