Detailed information of TA system TA225011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1497557..1498179
• Replicon: chromosome
• Accession: NZ_CP087177
• Organism: Pseudomonas moraviensis strain B21-039

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY57_RS06505
• Protein ID: WP_082367752.1
• Coordinates: 1498000..1498179 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2U1RCR8
• Locus tag: LOY57_RS06500
• Protein ID: WP_064362606.1
• Coordinates: 1497557..1497964 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY57_RS06470 (LOY57_06460)	1493043..1494128	+	1086	WP_083353380.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
LOY57_RS06475 (LOY57_06465)	1494237..1495007	+	771	WP_258640727.1	trans-aconitate 2-methyltransferase	-
LOY57_RS06480 (LOY57_06470)	1495138..1495866	-	729	WP_064362611.1	class I SAM-dependent methyltransferase	-
LOY57_RS06485 (LOY57_06475)	1496296..1496655	+	360	WP_039760852.1	DUF6124 family protein	-
LOY57_RS06490 (LOY57_06480)	1496723..1497079	-	357	WP_042608155.1	transcriptional regulator	-
LOY57_RS06495 (LOY57_06485)	1497079..1497381	-	303	WP_065615204.1	type II toxin-antitoxin system HigB family toxin	-
LOY57_RS06500 (LOY57_06490)	1497557..1497964	-	408	WP_064362606.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY57_RS06505 (LOY57_06495)	1498000..1498179	-	180	WP_082367752.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY57_RS06515 (LOY57_06505)	1498849..1499733	-	885	WP_258640731.1	alpha/beta hydrolase	-
LOY57_RS06520 (LOY57_06510)	1500050..1500742	-	693	WP_083353378.1	16S rRNA pseudouridine(516) synthase	-
LOY57_RS06525 (LOY57_06515)	1500776..1501006	-	231	WP_064362599.1	cysteine-rich CWC family protein	-
LOY57_RS06530 (LOY57_06520)	1500999..1502486	-	1488	WP_042608152.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1491740..1498179	6439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6662.95 Da
• Isoelectric Point: 11.8222

>T225011 WP_082367752.1 NZ_CP087177:c1498179-1498000 [Pseudomonas moraviensis]
VNSRFLIGQLVADGWFLVRIRGSHHHFKHPTKPGLVTVPHPKKDLLKKTAVSILQQAML

Nucleotide

• Length: 180 bp

>T225011 NZ_CP120837:310521-310889 [Salmonella enterica subsp. enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14567.59 Da
• Isoelectric Point: 4.5117

>AT225011 WP_064362606.1 NZ_CP087177:c1497964-1497557 [Pseudomonas moraviensis]
MLYPIAISIGDEEHAWGVEVPDLPGCFSAGEDLDDAVAMAREAIEGHFEILADDDAPIPPASKLSLHVANPKYAGCAWAV
VDIDVVKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT225011 NZ_CP120837:310303-310524 [Salmonella enterica subsp. enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2U1RCR8