Detailed information of TA system TA225009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 182150..182666
• Replicon: chromosome
• Accession: NZ_CP087177
• Organism: Pseudomonas moraviensis strain B21-039

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY57_RS00770
• Protein ID: WP_042610152.1
• Coordinates: 182385..182666 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2U1R4X8
• Locus tag: LOY57_RS00765
• Protein ID: WP_041477418.1
• Coordinates: 182150..182395 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY57_RS00745 (LOY57_00745)	177595..179133	+	1539	WP_258639940.1	MFS transporter	-
LOY57_RS00750 (LOY57_00750)	179162..180229	+	1068	WP_258639942.1	HlyD family secretion protein	-
LOY57_RS00755 (LOY57_00755)	180226..181794	+	1569	WP_258639944.1	efflux transporter outer membrane subunit	-
LOY57_RS00760 (LOY57_00760)	181873..182112	+	240	WP_064361654.1	DUF2789 domain-containing protein	-
LOY57_RS00765 (LOY57_00765)	182150..182395	+	246	WP_041477418.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOY57_RS00770 (LOY57_00770)	182385..182666	+	282	WP_042610152.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY57_RS00775 (LOY57_00775)	183031..183459	+	429	WP_016772266.1	winged helix DNA-binding protein	-
LOY57_RS00780 (LOY57_00780)	183456..185519	+	2064	WP_258639948.1	FUSC family protein	-
LOY57_RS00785 (LOY57_00785)	185516..185725	+	210	WP_016772264.1	DUF1656 domain-containing protein	-
LOY57_RS00790 (LOY57_00790)	185722..186609	+	888	WP_227973411.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10814.71 Da
• Isoelectric Point: 10.5399

>T225009 WP_042610152.1 NZ_CP087177:182385-182666 [Pseudomonas moraviensis]
MTYKLEFLPSAHKEWNKLGHTLREQFKKKLGERLKLPRIPADALHSMPDCYKIKLKASGYRLVYQVIDERVVVSVLAVGK
RERSSVYDAAKNR

Nucleotide

• Length: 282 bp

>T225009 NZ_CP120736:1341713-1342060 [Lacticaseibacillus rhamnosus]
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8806.13 Da
• Isoelectric Point: 4.4988

>AT225009 WP_041477418.1 NZ_CP087177:182150-182395 [Pseudomonas moraviensis]
MQSVLADMAVSVSELKKNPTAVLNGAHGGAVAVLNHNRVMGYMVPAEVYEAMMERLDDLELADIVRSRLHETPISVSLDD
L

Nucleotide

• Length: 246 bp

>AT225009 NZ_CP120736:1341495-1341713 [Lacticaseibacillus rhamnosus]
ATGATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGA
GGCGTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAAC
CATTATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2U1R4X8