Detailed information of TA system TA225006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2934823..2935342
• Replicon: chromosome
• Accession: NZ_CP087176
• Organism: Pseudomonas sp. B21-040

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY55_RS13630
• Protein ID: WP_077430244.1
• Coordinates: 2935061..2935342 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6L5C3D1
• Locus tag: LOY55_RS13625
• Protein ID: WP_046027440.1
• Coordinates: 2934823..2935071 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY55_RS13600 (LOY55_13580)	2930741..2931151	+	411	WP_046027445.1	MerR family transcriptional regulator	-
LOY55_RS13605 (LOY55_13585)	2931142..2931399	-	258	WP_046027444.1	DUF2790 domain-containing protein	-
LOY55_RS13610 (LOY55_13590)	2931463..2933010	-	1548	WP_258668048.1	MFS transporter	-
LOY55_RS13615 (LOY55_13595)	2933114..2933980	+	867	WP_258668049.1	LysR substrate-binding domain-containing protein	-
LOY55_RS13620 (LOY55_13600)	2934047..2934490	-	444	WP_109784713.1	thioredoxin family protein	-
LOY55_RS13625 (LOY55_13605)	2934823..2935071	+	249	WP_046027440.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOY55_RS13630 (LOY55_13610)	2935061..2935342	+	282	WP_077430244.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY55_RS13635 (LOY55_13615)	2935284..2935510	-	227	Protein_2679	5'-nucleotidase	-
LOY55_RS13640 (LOY55_13620)	2935757..2936605	+	849	WP_077430243.1	transporter substrate-binding domain-containing protein	-
LOY55_RS13645 (LOY55_13625)	2936658..2937320	+	663	WP_109784714.1	amino acid ABC transporter permease	-
LOY55_RS13650 (LOY55_13630)	2937329..2937991	+	663	WP_109784715.1	amino acid ABC transporter permease	-
LOY55_RS13655 (LOY55_13635)	2937988..2938779	+	792	WP_109784716.1	amino acid ABC transporter ATP-binding protein	-
LOY55_RS13660 (LOY55_13640)	2938824..2939213	+	390	WP_046027435.1	RidA family protein	-
LOY55_RS13665 (LOY55_13645)	2939371..2940135	+	765	WP_223524763.1	IclR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10852.58 Da
• Isoelectric Point: 10.4983

>T225006 WP_077430244.1 NZ_CP087176:2935061-2935342 [Pseudomonas sp. B21-040]
MTYELEFSEKAWKEWQKLGGNLREQFKRKLQERLLNPHVPADRLSGLGNAYKIKLRGAGYRMVYRVKDEVLIVTVIAVGK
RERGDVYKNAGDR

Nucleotide

• Length: 282 bp

>T225006 NZ_CP120736:331775-331960 [Lacticaseibacillus rhamnosus]
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8949.40 Da
• Isoelectric Point: 4.5338

>AT225006 WP_046027440.1 NZ_CP087176:2934823-2935071 [Pseudomonas sp. B21-040]
MTHIVLSQVVASISELKKNPMGTVAAGGGLSVAILNRNEPAFYCVPAKEYEAMMSRLEDLELIALCKERENDPTIKVSID
DL

Nucleotide

• Length: 249 bp

>AT225006 NZ_CP120736:331475-331747 [Lacticaseibacillus rhamnosus]
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTCCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A6L5C3D1