Detailed information of TA system TA225005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 228741..229384
• Replicon: chromosome
• Accession: NZ_CP087176
• Organism: Pseudomonas sp. B21-040

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2V2AQ77
• Locus tag: LOY55_RS01050
• Protein ID: WP_046031318.1
• Coordinates: 228741..228923 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY55_RS01055
• Protein ID: WP_046031317.1
• Coordinates: 228983..229384 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY55_RS01030 (LOY55_01030)	224542..226503	+	1962	WP_223523169.1	choline transporter BetT	-
LOY55_RS01035 (LOY55_01035)	226531..226791	-	261	WP_046031321.1	hypothetical protein	-
LOY55_RS01040 (LOY55_01040)	226865..227599	-	735	WP_223523131.1	SDR family oxidoreductase	-
LOY55_RS01045 (LOY55_01045)	227722..228612	+	891	WP_223523133.1	LysR family transcriptional regulator	-
LOY55_RS01050 (LOY55_01050)	228741..228923	+	183	WP_046031318.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY55_RS01055 (LOY55_01055)	228983..229384	+	402	WP_046031317.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY55_RS01060 (LOY55_01060)	229520..230557	+	1038	WP_223523135.1	L-glyceraldehyde 3-phosphate reductase	-
LOY55_RS01065 (LOY55_01065)	230690..231532	-	843	WP_109785294.1	taurine dioxygenase	-
LOY55_RS01070 (LOY55_01070)	231677..232510	-	834	WP_077432247.1	taurine ABC transporter permease TauC	-
LOY55_RS01075 (LOY55_01075)	232507..233301	-	795	WP_046031313.1	taurine ABC transporter ATP-binding subunit	-
LOY55_RS01080 (LOY55_01080)	233318..234295	-	978	WP_046031312.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6949.09 Da
• Isoelectric Point: 10.3955

>T225005 WP_046031318.1 NZ_CP087176:228741-228923 [Pseudomonas sp. B21-040]
MRSREMIRMIEEDGWYLVAVKGSHHQYKHLYKSGRVTIKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T225005 NZ_CP120733:191871-192233 [Tepidibacter sp. SWIR-1]
GTGAATAAAAATTTAGAGATTAAACGAGGTGACATATTTTACGGCGACTTAAGTCCTGTAATAGGGTCAGAACAAGGGGG
AGTAAGACCCGTATTGATTATTCAAAACGATATAGGCAACAAATACAGCCCAACAGTTATAATTGCAGCTATAACGTCTC
AAATCAACAAGGCAAAATTACCGACTCACATTGAAATAAATGCGAATGATTACGGACTTAATAGGGACTCAGTGGTATTA
CTAGAACAGGTAAGAACCATAGATAAAAAGAGATTGAGAGAAAAAATAGGCAATTTCGATGAGGATATGATGAAAAAAGT
AGACGAGGGTCTTCAAATAAGTTTAGGATTGTTTGATATTTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14541.31 Da
• Isoelectric Point: 4.4829

>AT225005 WP_046031317.1 NZ_CP087176:228983-229384 [Pseudomonas sp. B21-040]
MKFPVVLHKDADSEYGVIIPDVPGCFSAGGTVAQAFENVKEALSLHYEGLVADGDPLPQVREIDAHLDNPDYAGGVWGVV
EFDITPYFGKSVRFNATLPEQLLERIDQTVSRDRRYSSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT225005 NZ_CP120733:191594-191878 [Tepidibacter sp. SWIR-1]
GTGTCTAGTTGTGTGGATAAAAAGAAAATCGTTGTAAGTTTACCTAATAGCTTACTTTCAGAAGTGGATAAAATAATAAA
AGTAGAAAACAAAAACAGATCAGAATTTATAAAAGAAGCTATGAACCTTTATTTGAGAGAAAAAAGAAAAGTTCAAACAA
GAGAATCTATGATAAAAGGTTATAGAGAAATGGGTGTTATAAACTTAGCTTTAGCAGAAATGGGTCTTAGCATTGATATG
TCTTCTTTAGAAGGGTATGAAGGGAAGATAGCGGAAGGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A2V2AQ77