Detailed information of TA system TA225004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3531727..3532246
• Replicon: chromosome
• Accession: NZ_CP087175
• Organism: Pseudomonas sp. B21-041

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY43_RS15700
• Protein ID: WP_150692275.1
• Coordinates: 3531727..3532008 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4R7V066
• Locus tag: LOY43_RS15705
• Protein ID: WP_038368375.1
• Coordinates: 3531998..3532246 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY43_RS15665 (LOY43_15630)	3526900..3527289	-	390	WP_007912330.1	RidA family protein	-
LOY43_RS15670 (LOY43_15635)	3527320..3528111	-	792	WP_258708332.1	amino acid ABC transporter ATP-binding protein	-
LOY43_RS15675 (LOY43_15640)	3528108..3528770	-	663	WP_095047813.1	amino acid ABC transporter permease	-
LOY43_RS15680 (LOY43_15645)	3528780..3529442	-	663	WP_003225455.1	amino acid ABC transporter permease	-
LOY43_RS15685 (LOY43_15650)	3529494..3530342	-	849	WP_258708333.1	transporter substrate-binding domain-containing protein	-
LOY43_RS15690 (LOY43_15655)	3530767..3531174	+	408	WP_258708334.1	hypothetical protein	-
LOY43_RS15695 (LOY43_15660)	3531267..3531698	+	432	WP_258708335.1	thioredoxin family protein	-
LOY43_RS15700 (LOY43_15665)	3531727..3532008	-	282	WP_150692275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY43_RS15705 (LOY43_15670)	3531998..3532246	-	249	WP_038368375.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOY43_RS15710 (LOY43_15675)	3532541..3534100	-	1560	WP_258708336.1	TerC family protein	-
LOY43_RS15715 (LOY43_15680)	3534492..3536138	-	1647	WP_258708337.1	phospholipase D-like domain-containing protein	-
LOY43_RS15720 (LOY43_15685)	3536314..3536976	-	663	WP_258709937.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10822.66 Da
• Isoelectric Point: 10.5377

>T225004 WP_150692275.1 NZ_CP087175:c3532008-3531727 [Pseudomonas sp. B21-041]
MTYELEFSEKAWKEWEKLGAGLRDQFKKKLAERLLNPHVPADRLKGLGNAYKIKLRSAGYRLVYRVKDEVLVVTVIAVGK
RERSQIYTEAAKR

Nucleotide

• Length: 282 bp

>T225004 NZ_CP120732:c804468-804220 [Burkholderia vietnamiensis]
ATGAAGACGACGATTCGCAGAATGGGCAACTCGCAGGGCGTGCTGATTCCGAAGCCCGTCCTTGCGCAACTCGGGCTGGA
GAGCGAAGTGGAAATGGAGGTCGAGAACGACGCGATTGTTCTGCGCCGCCCGAAGCACAAAGCCCGTGAGGGGTGGGCGG
AAGCAAGCAAGGCTCTGGCCGAGAGCGGCGACGATGCGCTGGTTATGGGCGAGTTTTCGAACGCCGATGACGCGGAGCTT
GCATGGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8971.41 Da
• Isoelectric Point: 5.0492

>AT225004 WP_038368375.1 NZ_CP087175:c3532246-3531998 [Pseudomonas sp. B21-041]
MAHIVLSHVVASISELKKNPMGTVAAGGGRSVAILNRNEPAFYCVPAKEYEAMMTRLDDLELIALCKERENDPTIKVSID
DL

Nucleotide

• Length: 249 bp

>AT225004 NZ_CP120732:c804226-803906 [Burkholderia vietnamiensis]
ATGGTGATGCGCGGGGAAGTCTGGTTGGTCGCACTCGATCCGACGCTTGGTAGCGAGATTCAAAAGACGCGCCCCTGTGT
GGTCGTTTCACCTCCCGAAATGCACGACCACTTGCGCACTGTCATCGTCGCGCCGATGACTTCCAAGGGGCGTCCAGTGC
CGTTTCGGATTCCGGTTACGTTCAAGCGCAAACATGGTTTGATCCTTCTGGATCAGATTCGCGCAGTGGACAAGGTGCGC
TTGGTTAAGAAAGAGGGTGCAGTTGCCGATAAAACGTTGTTGGATACCCTGCGGACCTTGCAGGAAGTCTTTGCGGAATA
A

Structures

Antitoxin

• AlphaFold DB: A0A4R7V066