Detailed information of TA system TA225001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1831368..1831984
• Replicon: chromosome
• Accession: NZ_CP087175
• Organism: Pseudomonas sp. B21-041

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY43_RS08150
• Protein ID: WP_095050205.1
• Coordinates: 1831368..1831580 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7Z2KRV3
• Locus tag: LOY43_RS08155
• Protein ID: WP_064390887.1
• Coordinates: 1831580..1831984 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY43_RS08125 (LOY43_08100)	1826543..1828531	+	1989	WP_220558332.1	heme lyase CcmF/NrfE family subunit	-
LOY43_RS08130 (LOY43_08105)	1828528..1829064	+	537	WP_093439831.1	DsbE family thiol:disulfide interchange protein	-
LOY43_RS08135 (LOY43_08110)	1829061..1829531	+	471	WP_095050203.1	cytochrome c-type biogenesis protein CcmH	-
LOY43_RS08140 (LOY43_08115)	1829528..1830730	+	1203	WP_095050204.1	c-type cytochrome biogenesis protein CcmI	-
LOY43_RS08145 (LOY43_08120)	1830756..1831160	+	405	WP_258709443.1	hypothetical protein	-
LOY43_RS08150 (LOY43_08125)	1831368..1831580	+	213	WP_095050205.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY43_RS08155 (LOY43_08130)	1831580..1831984	+	405	WP_064390887.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY43_RS08160 (LOY43_08135)	1832054..1833241	-	1188	WP_258709444.1	MFS transporter	-
LOY43_RS08165 (LOY43_08140)	1833483..1834103	+	621	WP_258709445.1	DUF5666 domain-containing protein	-
LOY43_RS08170 (LOY43_08145)	1834342..1835340	-	999	WP_095126517.1	sulfate ABC transporter substrate-binding protein	-
LOY43_RS08175 (LOY43_08150)	1835542..1836366	-	825	WP_007919942.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8044.22 Da
• Isoelectric Point: 10.3456

>T225001 WP_095050205.1 NZ_CP087175:1831368-1831580 [Pseudomonas sp. B21-041]
VQSRLLIKELEEAGWTLDRITGSHHIFRHRYNPYTIPVPHPKKDLPLGTVKSIRRRAGLYNPPASFEGDP

Nucleotide

• Length: 213 bp

>T225001 NZ_CP120731:c184146-183808 [Burkholderia vietnamiensis]
ATGGTTGACATATCGAGTAACGTGCCTTACAGTGCTCCGACGTACAGCATCAGAACGACCGAAGTCTTCGACACATGGTT
CGCCGGTCTGCAGGATCGCGTCGCACGGCGGCGTATCCAGGCCCGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAGTCGGCCGGCTCCCCGGTCGTCGAGATGCGTATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCAGGCGCGCG
ACGATCTGGGTGATCCTGCTCTGCGGCGGCGACAAGTCGACGCAACAGGCAGACATTCGCGCCGCGCACGCAATGCTCGG
GCGGCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14789.75 Da
• Isoelectric Point: 4.3849

>AT225001 WP_064390887.1 NZ_CP087175:1831580-1831984 [Pseudomonas sp. B21-041]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDSFEDAYKAAVEVAHIMLQEIAADGESIPMPTSASAHRSNPDFADMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT225001 NZ_CP120731:c183804-183505 [Burkholderia vietnamiensis]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGCCCACCTCGAAACCGACGACGACATCGCCGACTATTTCGACGC
GTGCCTGCAGGAAGGCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCGCGCGCGCGCGGCATGTCGC
AGGTCGCACGCGACGCCGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACC
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCGCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z2KRV3