Detailed information of TA system TA225000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1530421..1531044
• Replicon: chromosome
• Accession: NZ_CP087175
• Organism: Pseudomonas sp. B21-041

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY43_RS06810
• Protein ID: WP_095049193.1
• Coordinates: 1530865..1531044 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: J2R7W3
• Locus tag: LOY43_RS06805
• Protein ID: WP_007911473.1
• Coordinates: 1530421..1530828 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY43_RS06775 (LOY43_06760)	1526288..1526443	+	156	WP_007911485.1	KGG domain-containing protein	-
LOY43_RS06780 (LOY43_06765)	1526520..1527023	+	504	WP_258709380.1	DUF4142 domain-containing protein	-
LOY43_RS06785 (LOY43_06770)	1527107..1527517	-	411	WP_007911483.1	low affinity iron permease family protein	-
LOY43_RS06790 (LOY43_06775)	1527682..1528452	+	771	WP_258709381.1	trans-aconitate 2-methyltransferase	-
LOY43_RS06795 (LOY43_06780)	1528690..1529418	-	729	WP_258709382.1	class I SAM-dependent methyltransferase	-
LOY43_RS06800 (LOY43_06785)	1529990..1530373	+	384	WP_258709383.1	DUF6124 family protein	-
LOY43_RS06805 (LOY43_06790)	1530421..1530828	-	408	WP_007911473.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY43_RS06810 (LOY43_06795)	1530865..1531044	-	180	WP_095049193.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY43_RS06815 (LOY43_06800)	1531410..1531928	+	519	WP_099758239.1	hypothetical protein	-
LOY43_RS06825 (LOY43_06810)	1532616..1533500	-	885	WP_095125591.1	alpha/beta hydrolase	-
LOY43_RS06830 (LOY43_06815)	1533817..1534509	-	693	WP_095049197.1	pseudouridine synthase	-
LOY43_RS06835 (LOY43_06820)	1534543..1534773	-	231	WP_064119704.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6646.93 Da
• Isoelectric Point: 11.1923

>T225000 WP_095049193.1 NZ_CP087175:c1531044-1530865 [Pseudomonas sp. B21-041]
VNSRFLIGQLVADGWYLVRIKGSHHHFKHPTKPGLVTVPHPKKDLLKKTAISILQQALL

Nucleotide

• Length: 180 bp

>T225000 NZ_CP120731:c183804-183505 [Burkholderia vietnamiensis]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGCCCACCTCGAAACCGACGACGACATCGCCGACTATTTCGACGC
GTGCCTGCAGGAAGGCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCGCGCGCGCGCGGCATGTCGC
AGGTCGCACGCGACGCCGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACC
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCGCGCGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14549.58 Da
• Isoelectric Point: 4.7324

>AT225000 WP_007911473.1 NZ_CP087175:c1530828-1530421 [Pseudomonas sp. B21-041]
MLYPIAISIGDDHHAWGVEVPDIPGCFSAGEDLDDAVAMAREAIEGHFEILADDDAPIPAASKLSLHVANPKYAGCAWAV
VDIDVLKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT225000 NZ_CP120731:c184146-183808 [Burkholderia vietnamiensis]
ATGGTTGACATATCGAGTAACGTGCCTTACAGTGCTCCGACGTACAGCATCAGAACGACCGAAGTCTTCGACACATGGTT
CGCCGGTCTGCAGGATCGCGTCGCACGGCGGCGTATCCAGGCCCGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAGTCGGCCGGCTCCCCGGTCGTCGAGATGCGTATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCAGGCGCGCG
ACGATCTGGGTGATCCTGCTCTGCGGCGGCGACAAGTCGACGCAACAGGCAGACATTCGCGCCGCGCACGCAATGCTCGG
GCGGCTCGACCTGGAGTAA

Structures

Antitoxin

• AlphaFold DB: J2R7W3