Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/upstrm_HI1419-dnstrm_HI1420
Location 984535..985129 Replicon chromosome
Accession NZ_CP087174
Organism Pseudomonas donghuensis strain B21-042

Toxin (Protein)


Gene name relE Uniprot ID A0A2M9VPP2
Locus tag LOY32_RS04455 Protein ID WP_036993896.1
Coordinates 984535..984828 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A2M9VPL5
Locus tag LOY32_RS04460 Protein ID WP_010223489.1
Coordinates 984830..985129 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY32_RS04435 (LOY32_04435) 981302..982273 - 972 WP_258694208.1 FecR family protein -
LOY32_RS04440 (LOY32_04440) 982266..982799 - 534 WP_010223492.1 RNA polymerase sigma factor -
LOY32_RS04445 (LOY32_04445) 982858..983538 - 681 WP_258694209.1 hypothetical protein -
LOY32_RS04450 (LOY32_04450) 983873..984109 - 237 WP_081492801.1 hypothetical protein -
LOY32_RS04455 (LOY32_04455) 984535..984828 + 294 WP_036993896.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOY32_RS04460 (LOY32_04460) 984830..985129 + 300 WP_010223489.1 putative addiction module antidote protein Antitoxin
LOY32_RS04465 (LOY32_04465) 985152..985778 - 627 Protein_875 effector protein -
LOY32_RS04470 (LOY32_04470) 985831..986307 - 477 WP_036993897.1 GNAT family N-acetyltransferase -
LOY32_RS04475 (LOY32_04475) 986447..986959 + 513 WP_258694210.1 superoxide dismutase [Cu-Zn] SodC -
LOY32_RS04480 (LOY32_04480) 987032..987337 + 306 WP_026001347.1 HigA family addiction module antitoxin -
LOY32_RS04490 (LOY32_04490) 987580..988629 + 1050 WP_010223475.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
LOY32_RS04495 (LOY32_04495) 988644..989759 + 1116 WP_010223474.1 tRNA guanosine(34) transglycosylase Tgt -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(5-97)

Antitoxin

Uncharacterized protein HI1420

(5-93)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10685.48 Da        Isoelectric Point: 10.7515

>T224998 WP_036993896.1 NZ_CP087174:984535-984828 [Pseudomonas donghuensis]
MKYSVLQTPRFTSWLISVRDMRARVAIARRIERVTAGNLGDVKSVGGQVCEMRIDVGAGYRVYFTARDGVVIVLLAGGDK
SSQSVDIQLARKLAKEI

Download         Length: 294 bp

>T224998 NZ_CP120723:c1386164-1385418 [Bacillus atrophaeus]
ATGCTTTTATTCTTTCAGATTATCGTTTGGTGCATAATGGCCGCATTAGGCCTATACGTATATGCGGTGTGGCGGTTTGA
AGCAAAGGTGAAAGAAAAAATGTTTGCGATCCGGAAAACGTGGTATTTGATCTTTGTGATCGGCGCCATGGTATACTGGA
CGTATGACCCCTCTTCCCTTTTCAGGGAATGGACGAATTATTTGATTGTCGCTGTCAGCTTTGCTTTGATTGACGCCTTT
ATCTTTTTGAGCGCTTACGTAAAGAAACTTGCCGGCAATGAATTAGAAACCGACACACGGGAAATCCTTGAAGAAAACAA
TGAAATGCTCCACGTTTATCTTGATAAATTAAAAACGTACCAATACCTATTGAAAAATGAACCGATCCATGTTTATTATG
GAAGTATAGAAGCATACGCCGAGGGCATCGACAAGCTGCTCAAAACCTATGCCGACAAAATGAACTTAACGGCTTCGCTG
TGTGAATATTCGACACAGCTAGAAAAAGACCGCTTAACCGAGCATATGGATGATGCATTTGAGGTGCAGAGCCGGCTGGA
TCGAAAAGATGTATACTATGATCAGCACGGGAAGCTGGCACTCATCCCTTTTACAGTCCAGACACGCAGCTACGTCATCA
AGCTGGCGTCAGACAGCATTGTAACAGAATTTGATTATTTGCTTTTCACGTCATTAACTAGCATTTACGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGACGGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10629.21 Da        Isoelectric Point: 6.2132

>AT224998 WP_010223489.1 NZ_CP087174:984830-985129 [Pseudomonas donghuensis]
MSLSLTTFDMAALLDSDETISEYLSQVLADGDHDELIRALGYIAKARGMSQIATASGLGRESLYKALTPGAKPRFDTVLK
VIRALGIDLLAHPHASSRL

Download         Length: 300 bp

>AT224998 NZ_CP120723:c1385418-1385248 [Bacillus atrophaeus]
ATGGAACGTGCTTTTCAAAACGATCATTCGCCCCGCGCGGCTAAACCGTTTAAGATATTAAAAAAGAGATCAATCACCAG
CGTCGCAAGCTATCAGGTCAGCCCCCACACGGCAAGAATTTTCAAAGAAAATGAGCGGCTGATTGACGAGTATAAAAGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2M9VPP2

Antitoxin

Source ID Structure
AlphaFold DB A0A2M9VPL5

References