Detailed information of TA system TA224997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 955767..956361
• Replicon: chromosome
• Accession: NZ_CP087173
• Organism: Pseudomonas donghuensis strain B21-043

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY30_RS04295
• Protein ID: WP_258702330.1
• Coordinates: 955767..956060 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A2M9VPL5
• Locus tag: LOY30_RS04300
• Protein ID: WP_010223489.1
• Coordinates: 956062..956361 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY30_RS04275 (LOY30_04275)	952534..953505	-	972	WP_258702329.1	FecR family protein	-
LOY30_RS04280 (LOY30_04280)	953498..954031	-	534	WP_010223492.1	RNA polymerase sigma factor	-
LOY30_RS04285 (LOY30_04285)	954090..954770	-	681	WP_145959264.1	hypothetical protein	-
LOY30_RS04290 (LOY30_04290)	955105..955341	-	237	WP_081492801.1	hypothetical protein	-
LOY30_RS04295 (LOY30_04295)	955767..956060	+	294	WP_258702330.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY30_RS04300 (LOY30_04300)	956062..956361	+	300	WP_010223489.1	putative addiction module antidote protein	Antitoxin
LOY30_RS04305 (LOY30_04305)	956384..957010	-	627	Protein_844	effector protein	-
LOY30_RS04310 (LOY30_04310)	957063..957539	-	477	WP_036993897.1	GNAT family N-acetyltransferase	-
LOY30_RS04315 (LOY30_04315)	957679..958191	+	513	WP_258702331.1	superoxide dismutase [Cu-Zn] SodC	-
LOY30_RS04320 (LOY30_04320)	958264..958569	+	306	WP_026001347.1	HigA family addiction module antitoxin	-
LOY30_RS04325 (LOY30_04325)	958905..959816	-	912	WP_258702332.1	LysR family transcriptional regulator	-
LOY30_RS04330 (LOY30_04330)	959956..960702	+	747	WP_258702333.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (7-97)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10651.46 Da
• Isoelectric Point: 10.7515

>T224997 WP_258702330.1 NZ_CP087173:955767-956060 [Pseudomonas donghuensis]
MKYSVLQTPRLTSWLISVRDMRARVAIARRIERVTAGNLGDVKSVGGQVCEMRIDVGAGYRVYFTARDGVVIVLLAGGDK
SSQSVDIQLARKLAKEI

Nucleotide

• Length: 294 bp

>T224997 NZ_CP120723:586935-587285 [Bacillus atrophaeus]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGAGTGCGCCCGGT
TTTGGTGATTCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATTTTGCTGGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATTAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10629.21 Da
• Isoelectric Point: 6.2132

>AT224997 WP_010223489.1 NZ_CP087173:956062-956361 [Pseudomonas donghuensis]
MSLSLTTFDMAALLDSDETISEYLSQVLADGDHDELIRALGYIAKARGMSQIATASGLGRESLYKALTPGAKPRFDTVLK
VIRALGIDLLAHPHASSRL

Nucleotide

• Length: 300 bp

>AT224997 NZ_CP120723:586649-586930 [Bacillus atrophaeus]
TTGTCTGAATCCAGCGCAACAACCGAAATGATGATTAGCTTGCCGGAAGCACTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTAATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGCACAACTCGTCACAACC
GTGATTTGATGAGACGCGGATATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAAGCTCATTTTGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A2M9VPL5