Detailed information of TA system TA224994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 5910832..5912456
• Replicon: chromosome
• Accession: NZ_CP087171
• Organism: Pseudomonas atacamensis strain B21-045

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LOY27_RS26690
• Protein ID: WP_258710753.1
• Coordinates: 5911107..5912456 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6I0CP94
• Locus tag: LOY27_RS26685
• Protein ID: WP_071171374.1
• Coordinates: 5910832..5911110 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY27_RS26655 (LOY27_26615)	5906272..5906895	+	624	WP_258604231.1	FMN-binding negative transcriptional regulator	-
LOY27_RS26660 (LOY27_26620)	5906906..5907352	+	447	WP_024014846.1	GNAT family N-acetyltransferase	-
LOY27_RS26665 (LOY27_26625)	5907365..5908036	+	672	WP_160767985.1	GNAT family protein	-
LOY27_RS26670 (LOY27_26630)	5908156..5909059	-	904	Protein_5243	homocysteine S-methyltransferase family protein	-
LOY27_RS26675 (LOY27_26635)	5909173..5909991	+	819	WP_016772458.1	ABC transporter substrate-binding protein	-
LOY27_RS26680 (LOY27_26640)	5909988..5910824	+	837	WP_130900264.1	amino acid ABC transporter permease	-
LOY27_RS26685 (LOY27_26645)	5910832..5911110	-	279	WP_071171374.1	helix-turn-helix transcriptional regulator	Antitoxin
LOY27_RS26690 (LOY27_26650)	5911107..5912456	-	1350	WP_258710753.1	HipA domain-containing protein	Toxin
LOY27_RS26695 (LOY27_26655)	5912825..5915062	+	2238	WP_258710754.1	autotransporter outer membrane beta-barrel domain-containing protein	-
LOY27_RS26700 (LOY27_26660)	5915274..5917082	-	1809	WP_258710755.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49876.45 Da
• Isoelectric Point: 5.0718

>T224994 WP_258710753.1 NZ_CP087171:c5912456-5911107 [Pseudomonas atacamensis]
MYNLTIQIFTAGQWHDAMILDFDDPEKGFESRCSFGYEADYLVENIELIGSPFSQAVSALYPLDWEGRRAKTPAFVHDIA
PAGAARRFLLARLGQERPAEISADLFLLGRSTPAPIGNMRIKESAEAVDERKPIGFKREEVISRDNRFLEYAYELGAAIG
GATGAGGEAPKLLLAENSAGLLYPDAVLDDEEVRQHWFVKFARNRGGQTDQDILRSEFHYYKALQTLGIETVAAEGLALE
EATKPSLWMRRFDRQVKDGAVSRFAVESIYSLAEVTTPGSAMNHLEVIRMLAGLWRKAGQASQIPGLVADYLRRDLINKI
LGNSDNHGRNTAIIRQENAFQLAPIYDLAPMVMDDEGVTRTTKWPRELERAGDVDWRGVCRVLADSADPHDSLAGLDASA
LFEGLRADARRLAALPDILAASGLPARTMNHPSVHLKNLEQRLKEWDLQ

Nucleotide

• Length: 1350 bp

>T224994 NZ_CP120721:11076-11438 [Vibrio coralliilyticus]
ATGAGGGAACAGCCTATTACGGTATTACAAAAGCTTCAAGATGTGGTCGGTAAGCAACACCGAATCGTTGTTTCAGCTAT
CACTTACCAAGAAATGCAGTATGGGCTGCTAGGCAAAAAAGCATCACCGAAACATGCTGTGCTTGTTGAAGAGTTTCTAA
AGCGCGTCGATGAGATTTTGCCTTGGGACAAATCAGCCGTTGATGCAACGACAGAAGTAAAGCGAGATCTAATGGCTAAG
GGGACACCAATTGGCAATAACGACACTGCCATTGCGGGTCATGCCATCGCTACGGGCTGTGTCCTTGTCACGAACAATAC
GAGAGAGTTTCAGCGCGTAGAAGGACTAAAACTCGAAGATTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10318.09 Da
• Isoelectric Point: 10.7364

>AT224994 WP_071171374.1 NZ_CP087171:c5911110-5910832 [Pseudomonas atacamensis]
MSLTVLERTALIESIQQSLAEGTLEIGDAVRRLRVEVTGLHQSQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGM
KMGVVRIRRDTI

Nucleotide

• Length: 279 bp

>AT224994 NZ_CP120721:10800-11033 [Vibrio coralliilyticus]
ATGAGAACAGTTTCTATTTTTAGAAATGGCAAGAATCAAACGATACGCTTGCCAAAGGACTTTGAGTTTGACGGAGTTAA
CGAGCTCGAAATACACAAAGAGGGAGATGTGATTACTTTGCGTCCAGTTCGGCCCGATTGGCTTTCATTTGGCAACCAAG
AAGAGGCTAATGATGACTTCATGGCAACCCGTGAAGATGGAATAACGGACGAAGGACGATTCAATTTTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A6I0CP94