Detailed information of TA system TA224991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1768848..1769446
• Replicon: chromosome
• Accession: NZ_CP087171
• Organism: Pseudomonas atacamensis strain B21-045

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY27_RS07810
• Protein ID: WP_258711237.1
• Coordinates: 1768848..1769042 (+)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY27_RS07815
• Protein ID: WP_130901292.1
• Coordinates: 1769042..1769446 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY27_RS07780 (LOY27_07760)	1764932..1765468	+	537	WP_024012128.1	DsbE family thiol:disulfide interchange protein	-
LOY27_RS07785 (LOY27_07765)	1765465..1765935	+	471	WP_016771041.1	cytochrome c-type biogenesis protein CcmH	-
LOY27_RS07790 (LOY27_07770)	1765932..1767131	+	1200	WP_258711236.1	c-type cytochrome biogenesis protein CcmI	-
LOY27_RS07795 (LOY27_07775)	1767157..1767561	+	405	WP_016771039.1	hypothetical protein	-
LOY27_RS07800 (LOY27_07780)	1767607..1768182	-	576	WP_076566634.1	PIN domain-containing protein	-
LOY27_RS07805 (LOY27_07785)	1768179..1768646	-	468	WP_160768170.1	helix-turn-helix domain-containing protein	-
LOY27_RS07810 (LOY27_07790)	1768848..1769042	+	195	WP_258711237.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY27_RS07815 (LOY27_07795)	1769042..1769446	+	405	WP_130901292.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY27_RS07820 (LOY27_07800)	1769559..1770758	-	1200	WP_122611605.1	MFS transporter	-
LOY27_RS07825 (LOY27_07805)	1770925..1771923	-	999	WP_258711238.1	sulfate ABC transporter substrate-binding protein	-
LOY27_RS07830 (LOY27_07810)	1772028..1772852	-	825	WP_039763624.1	ion transporter	-
LOY27_RS07835 (LOY27_07815)	1772874..1773788	-	915	WP_258711239.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-53)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7313.34 Da
• Isoelectric Point: 10.2204

>T224991 WP_258711237.1 NZ_CP087171:1768848-1769042 [Pseudomonas atacamensis]
MKELEEAGWTLDRVAGNHHIFTHRYYPNTIAVPHPKKDLPLGTVKSIRRRAGLFSPPTRHEGDP

Nucleotide

• Length: 195 bp

>T224991 NZ_CP120711:3033815-3034165 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14804.86 Da
• Isoelectric Point: 4.5789

>AT224991 WP_130901292.1 NZ_CP087171:1769042-1769446 [Pseudomonas atacamensis]
MQYPICIEWGDENTAIGIQIPDIPGAVTVGDSFEEAYNAAVEVAHLMLQEIAAAGQAIPMPTSAAAHRNHPDFAEMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT224991 NZ_CP120711:3033528-3033809 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA