Detailed information of TA system TA224990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1767607..1768646
• Replicon: chromosome
• Accession: NZ_CP087171
• Organism: Pseudomonas atacamensis strain B21-045

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY27_RS07800
• Protein ID: WP_076566634.1
• Coordinates: 1767607..1768182 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LOY27_RS07805
• Protein ID: WP_160768170.1
• Coordinates: 1768179..1768646 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY27_RS07775 (LOY27_07755)	1762947..1764935	+	1989	WP_041479622.1	heme lyase CcmF/NrfE family subunit	-
LOY27_RS07780 (LOY27_07760)	1764932..1765468	+	537	WP_024012128.1	DsbE family thiol:disulfide interchange protein	-
LOY27_RS07785 (LOY27_07765)	1765465..1765935	+	471	WP_016771041.1	cytochrome c-type biogenesis protein CcmH	-
LOY27_RS07790 (LOY27_07770)	1765932..1767131	+	1200	WP_258711236.1	c-type cytochrome biogenesis protein CcmI	-
LOY27_RS07795 (LOY27_07775)	1767157..1767561	+	405	WP_016771039.1	hypothetical protein	-
LOY27_RS07800 (LOY27_07780)	1767607..1768182	-	576	WP_076566634.1	PIN domain-containing protein	Toxin
LOY27_RS07805 (LOY27_07785)	1768179..1768646	-	468	WP_160768170.1	helix-turn-helix domain-containing protein	Antitoxin
LOY27_RS07810 (LOY27_07790)	1768848..1769042	+	195	WP_258711237.1	type II toxin-antitoxin system HicA family toxin	-
LOY27_RS07815 (LOY27_07795)	1769042..1769446	+	405	WP_130901292.1	type II toxin-antitoxin system HicB family antitoxin	-
LOY27_RS07820 (LOY27_07800)	1769559..1770758	-	1200	WP_122611605.1	MFS transporter	-
LOY27_RS07825 (LOY27_07805)	1770925..1771923	-	999	WP_258711238.1	sulfate ABC transporter substrate-binding protein	-
LOY27_RS07830 (LOY27_07810)	1772028..1772852	-	825	WP_039763624.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21688.71 Da
• Isoelectric Point: 6.1390

>T224990 WP_076566634.1 NZ_CP087171:c1768182-1767607 [Pseudomonas atacamensis]
VRHSSFTAVYDACVLYPAPLRDFLMWLALSGLYRARWTQEIHHEWKRNLLKNRSDLTLEQLDRTSQLMDLAIPDACIDGY
EDLVSGLTLPDENDRHVLAAAIRCGADVIVTFNLKDFPTRHLAPYGIEAQHPDEFVENLFHLDSAAVVAAAQSQRRQLKT
PNVEVDKYLGFLQRQGLVESVKALGNYRAIL

Nucleotide

• Length: 576 bp

>T224990 NZ_CP120688:c2737343-2737125 [Lacticaseibacillus sp. DSM 115424]
ATCAGTTGGTTTGAGCAAGCTGATAAAGCCACGCTCAGAAAAATTAATCGGCTCATCACATCGATCGACCGGACACCGTT
TGATAGCCTGGGTAAACCAGAACCATTGAAACACGGCTTGTCTGGTATGTGGTCGCGCCGAATCACGCAAGAACACCGCT
TAATTTACGAAATTGAGGCAAACACGATGACAATTATTTCCTGTCGCGATCATTACTAG

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17253.67 Da
• Isoelectric Point: 6.6497

>AT224990 WP_160768170.1 NZ_CP087171:c1768646-1768179 [Pseudomonas atacamensis]
MTTATPSAINLPVEREVKAAIEGQRALSAFLTTAFETQRIQIFDEQNEAHSVELPTSALRLLSDILAALAQGNAVKVVPI
HAELTTQEAADLLNVSRPHMIKLLERGEISYHKTGKHRRVRFADLMEYKTRRDTASEQAMTLLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT224990 NZ_CP120688:c2737650-2737375 [Lacticaseibacillus sp. DSM 115424]
ATGGACGCTGTTTCCTACTCAGTTTTCCGCCGCAATCTCAAGCACTACCTTCAAGCAGTCAATGACACCTCCACGCCACT
CTTGATTACTAGCCGTGATAATGAGGCTGATACTGCCGTCTTGATGAATAAACGTGATTTTGATGCCATGCAAGAAACGA
TGAGGATTTTGTCGAATCCTGAACTCATGGCCAAGATCCGCCGTGGTCAAGCCCAAATTGCCGCTGGTAAAACCCGGCAA
CACGATTTAAAGGATGTCGGTGGCGGGGATGATTAA