Detailed information of TA system TA022499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 1011234..1011815
• Replicon: chromosome
• Accession: NC_009925
• Organism: Acaryochloris marina MBIC11017

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B0C1R4
• Locus tag: AM1_RS04615
• Protein ID: WP_012161638.1
• Coordinates: 1011234..1011518 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0C1R5
• Locus tag: AM1_RS04620
• Protein ID: WP_012161639.1
• Coordinates: 1011522..1011815 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM1_RS04610	1007828..1010539	+	2712	WP_012161636.1	IS66 family transposase	-
AM1_RS04615	1011234..1011518	+	285	WP_012161638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM1_RS04620	1011522..1011815	+	294	WP_012161639.1	putative addiction module antidote protein	Antitoxin
AM1_RS04625	1012072..1012635	+	564	WP_012161640.1	Uma2 family endonuclease	-
AM1_RS04630	1012726..1012944	+	219	WP_012161641.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AM1_RS04635	1012947..1013351	+	405	WP_041659114.1	type II toxin-antitoxin system VapC family toxin	-
AM1_RS42015	1013494..1013658	+	165	WP_012161643.1	hypothetical protein	-
AM1_RS04640	1013667..1014251	+	585	WP_012161644.1	DJ-1/PfpI family protein	-
AM1_RS04645	1014320..1014754	+	435	WP_012161645.1	GNAT family N-acetyltransferase	-
AM1_RS38470	1015504..1015695	+	192	WP_083776756.1	hypothetical protein	-
AM1_RS04655	1015972..1016484	+	513	WP_010467654.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1016778..1017833	1055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-93)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10737.30 Da
• Isoelectric Point: 9.5338

>T22499 WP_012161638.1 NC_009925:1011234-1011518 [Acaryochloris marina MBIC11017]
MIEVRQTDIFANWFNKLRDRKAKARIDRIEIGNFGDVAPIGEDVSELRMHYGSGYRVYFIQRGLVVVILIPGGDKGSQNA
DIKRAKEISKQLED

Nucleotide

• Length: 285 bp

>T22499 NC_009925:1011234-1011518 [Acaryochloris marina MBIC11017]
ATGATTGAGGTCCGTCAAACAGACATTTTTGCCAATTGGTTCAATAAACTGCGAGATCGAAAGGCTAAAGCACGTATTGA
CCGTATCGAGATTGGCAATTTTGGGGATGTAGCCCCCATCGGGGAAGATGTTAGCGAACTTCGCATGCATTATGGTTCTG
GTTACAGGGTTTACTTTATACAACGTGGTCTAGTGGTTGTCATATTGATTCCTGGTGGTGACAAAGGCTCACAAAATGCT
GACATCAAAAGGGCTAAGGAAATTTCTAAGCAGTTGGAGGATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10681.19 Da
• Isoelectric Point: 5.1131

>AT22499 WP_012161639.1 NC_009925:1011522-1011815 [Acaryochloris marina MBIC11017]
MPINTTRWDSAEHLKTEEDIQLYLEACLEEAEDDPALIIHALGVVARAKNMSQLSRDTGLSREGLYKALSPDGNPKFSTI
AKVTKALGFKLTIQPTP

Nucleotide

• Length: 294 bp

>AT22499 NC_009925:1011522-1011815 [Acaryochloris marina MBIC11017]
ATGCCCATAAACACAACCCGCTGGGATTCAGCGGAACATCTCAAAACAGAAGAAGATATTCAGCTATATCTAGAAGCATG
CCTGGAAGAAGCCGAAGATGATCCTGCTCTAATTATTCATGCACTAGGAGTAGTCGCTAGGGCTAAGAATATGAGTCAAC
TATCCCGAGATACGGGTTTGAGTAGAGAAGGGCTATATAAAGCGCTGTCTCCTGATGGTAACCCCAAATTTTCGACGATC
GCTAAAGTTACTAAAGCGCTCGGATTCAAGCTAACCATTCAGCCAACACCTTAG

Structures

Toxin

• AlphaFold DB: B0C1R4

Antitoxin

• AlphaFold DB: B0C1R5