Detailed information of TA system TA224989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 871734..872323
• Replicon: chromosome
• Accession: NZ_CP087171
• Organism: Pseudomonas atacamensis strain B21-045

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY27_RS03700
• Protein ID: WP_073473851.1
• Coordinates: 872072..872323 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V8R847
• Locus tag: LOY27_RS03695
• Protein ID: WP_016771749.1
• Coordinates: 871734..872075 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY27_RS03670 (LOY27_03660)	867437..867838	+	402	WP_016771753.1	30S ribosome-binding factor RbfA	-
LOY27_RS03675 (LOY27_03665)	867843..868760	+	918	WP_160767822.1	tRNA pseudouridine(55) synthase TruB	-
LOY27_RS03680 (LOY27_03670)	868904..869173	+	270	WP_016771751.1	30S ribosomal protein S15	-
LOY27_RS03685 (LOY27_03675)	869378..871483	+	2106	WP_024011572.1	polyribonucleotide nucleotidyltransferase	-
LOY27_RS03695 (LOY27_03685)	871734..872075	-	342	WP_016771749.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY27_RS03700 (LOY27_03690)	872072..872323	-	252	WP_073473851.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY27_RS03705 (LOY27_03695)	872679..872978	+	300	WP_042608866.1	DUF6388 family protein	-
LOY27_RS03710 (LOY27_03700)	873066..873914	-	849	WP_016771747.1	carboxylating nicotinate-nucleotide diphosphorylase	-
LOY27_RS03715 (LOY27_03705)	874182..876359	+	2178	WP_258710990.1	DUF1631 domain-containing protein	-
LOY27_RS03720 (LOY27_03710)	876424..876984	+	561	WP_122699435.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9241.67 Da
• Isoelectric Point: 10.4725

>T224989 WP_073473851.1 NZ_CP087171:c872323-872072 [Pseudomonas atacamensis]
MSKTEKLLAKLINGHSAFTWSELVTLLGRLGYSQLEGSGSRIKFDNGDPNALINLHKPHPGNEIKTYARRQIIEQLKKGG
LIQ

Nucleotide

• Length: 252 bp

>T224989 NZ_CP120688:c2626305-2625922 [Lacticaseibacillus sp. DSM 115424]
ATGGATGTAACCGTAAAACGCGGAGATGTCTTCTTCGCCGATTTATCGCCGGTTGTCGGATCCGAACAGGGTGGCAATCG
ACCTGTACTTATTATTCAAAACAACGTTGGCAACCACTATAGTCCTACGGTTATTGTGGCGGCCATTACCTCCAAGATCC
AAAAACCCAAGATGCCCACTCATGTTGGCTTACGGGCAAAGCAAGACGGGGTTGAACGTAACTCGGTGATTTTGCTAGAA
CAGATTCGCACCATCGATAAGCAGCGATTACAGGCGCGCGTGACAGCTTTATCAAGCGCAAAAATGGCGGCCGTTGATCG
TGCGTTGGCAATTTCAGTAGGGCTCGTTAGTTTGCCCAAACCAAAAACATATAATAAAAATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12594.21 Da
• Isoelectric Point: 5.9655

>AT224989 WP_016771749.1 NZ_CP087171:c872075-871734 [Pseudomonas atacamensis]
MSSQLHYKGYVGSIEASTEDKCLFGKLLFIRALVNYEGQTVAELEAAFREAVDDYLQTCEQLGQKPEIPCKGSFNVRIGH
DLHLAAALAATRQNVSLNDLTRQALNEYLQHHA

Nucleotide

• Length: 342 bp

>AT224989 NZ_CP120688:c2626573-2626325 [Lacticaseibacillus sp. DSM 115424]
ATGAAGAAGCAAACGTCAGTAACGGTAACGTTTGATGCTGCTATTAAGGCGCGGTTAGATGCCTATTGTGAGTTGAATGA
CACGGATACTCAAGATGTGATCACAACAGCAGTGGCCAAATTTTTGCAGTCACAAGCACCCGATATCAACCAGCTGATTT
CCGGCTATGTTGCCATGGGACAGATCAATACAGAGATCAGTCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Antitoxin

• AlphaFold DB: V8R847