Detailed information of TA system TA224986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 173790..174306
• Replicon: chromosome
• Accession: NZ_CP087171
• Organism: Pseudomonas atacamensis strain B21-045

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY27_RS00740
• Protein ID: WP_071174010.1
• Coordinates: 174025..174306 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2U1R4X8
• Locus tag: LOY27_RS00735
• Protein ID: WP_041477418.1
• Coordinates: 173790..174035 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY27_RS00715 (LOY27_00715)	169178..170716	+	1539	WP_258710803.1	MFS transporter	-
LOY27_RS00720 (LOY27_00720)	170744..171811	+	1068	WP_110602297.1	HlyD family secretion protein	-
LOY27_RS00725 (LOY27_00725)	171808..173376	+	1569	WP_258710804.1	efflux transporter outer membrane subunit	-
LOY27_RS00730 (LOY27_00730)	173455..173700	+	246	WP_125925865.1	DUF2789 domain-containing protein	-
LOY27_RS00735 (LOY27_00735)	173790..174035	+	246	WP_041477418.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOY27_RS00740 (LOY27_00740)	174025..174306	+	282	WP_071174010.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY27_RS00745 (LOY27_00745)	174670..175098	+	429	WP_016772266.1	winged helix DNA-binding protein	-
LOY27_RS00750 (LOY27_00750)	175095..177158	+	2064	WP_258710805.1	FUSC family protein	-
LOY27_RS00755 (LOY27_00755)	177155..177364	+	210	WP_016772264.1	DUF1656 domain-containing protein	-
LOY27_RS00760 (LOY27_00760)	177361..178248	+	888	WP_122698862.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10842.73 Da
• Isoelectric Point: 10.5812

>T224986 WP_071174010.1 NZ_CP087171:174025-174306 [Pseudomonas atacamensis]
MTYKLEFLPSAHKEWNKLGHTLREQFKKKLGERLKLPRIPADALHSMPDCYKIKLKASGYRLVYQVIDERVVVSVLAVGK
RERSSVYDAARNR

Nucleotide

• Length: 282 bp

>T224986 NZ_CP120681:4099323-4099673 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8806.13 Da
• Isoelectric Point: 4.4988

>AT224986 WP_041477418.1 NZ_CP087171:173790-174035 [Pseudomonas atacamensis]
MQSVLADMAVSVSELKKNPTAVLNGAHGGAVAVLNHNRVMGYMVPAEVYEAMMERLDDLELADIVRSRLHETPISVSLDD
L

Nucleotide

• Length: 246 bp

>AT224986 NZ_CP120681:4099037-4099318 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A2U1R4X8