Detailed information of TA system TA224973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 5859039..5860663
• Replicon: chromosome
• Accession: NZ_CP087167
• Organism: Pseudomonas atacamensis strain B21-050

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LOY49_RS26430
• Protein ID: WP_258604233.1
• Coordinates: 5859314..5860663 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6I0CP94
• Locus tag: LOY49_RS26425
• Protein ID: WP_071171374.1
• Coordinates: 5859039..5859317 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY49_RS26395 (LOY49_26365)	5854480..5855103	+	624	WP_258604231.1	FMN-binding negative transcriptional regulator	-
LOY49_RS26400 (LOY49_26370)	5855114..5855560	+	447	WP_024014846.1	GNAT family N-acetyltransferase	-
LOY49_RS26405 (LOY49_26375)	5855573..5856244	+	672	WP_160767985.1	GNAT family protein	-
LOY49_RS26410 (LOY49_26380)	5856364..5857266	-	903	WP_024014847.1	homocysteine S-methyltransferase family protein	-
LOY49_RS26415 (LOY49_26385)	5857380..5858198	+	819	WP_016772458.1	ABC transporter substrate-binding protein	-
LOY49_RS26420 (LOY49_26390)	5858195..5859031	+	837	WP_071171375.1	amino acid ABC transporter permease	-
LOY49_RS26425 (LOY49_26395)	5859039..5859317	-	279	WP_071171374.1	helix-turn-helix transcriptional regulator	Antitoxin
LOY49_RS26430 (LOY49_26400)	5859314..5860663	-	1350	WP_258604233.1	HipA domain-containing protein	Toxin
LOY49_RS26435 (LOY49_26405)	5861032..5863269	+	2238	WP_258604234.1	autotransporter outer membrane beta-barrel domain-containing protein	-
LOY49_RS26440 (LOY49_26410)	5863480..5865288	-	1809	WP_122506268.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49790.36 Da
• Isoelectric Point: 5.0663

>T224973 WP_258604233.1 NZ_CP087167:c5860663-5859314 [Pseudomonas atacamensis]
MYNLTIQIFTAGQWHDAMILDFDDPEKGFESRCSFGYEADYLVENIELIGSPFSQAVSALYPLDWEGRRAKTPAFVHDIA
PAGAAKRFLLARLGQERPAEISADLFLLGRSTPAPIGNMRIKESAEAVDERKPIGFKREEVIGRDNRFLEYAYELGAAIG
GATGAGGEAPKLLLAENSAGLLYPDAVLDDEEVRQHWFVKFARNRGGQTDQDILRSEFHYYKALQTLGIETVAAEGLALE
EATKPSLWMRRFDRQVKDGAVSRFAVESIYSLAEVTTPGSAMNHLDVIRMLAGLWRKAGQASQIPGLVADYLRRDLINKI
LGNSDNHGRNTAIIRQENAFQLAPIYDLAPMVMDDEGVTRTTKWPRELERAGDVDWRGVCRVLADSADPHDSLAGLDASA
LFDGLRADARRLAALPDILAASGLPARTMNHPSVHLKNLEQRLKEWDLQ

Nucleotide

• Length: 1350 bp

>T224973 NZ_CP120671:c3117663-3117445 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTATCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10318.09 Da
• Isoelectric Point: 10.7364

>AT224973 WP_071171374.1 NZ_CP087167:c5859317-5859039 [Pseudomonas atacamensis]
MSLTVLERTALIESIQQSLAEGTLEIGDAVRRLRVEVTGLHQSQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGM
KMGVVRIRRDTI

Nucleotide

• Length: 279 bp

>AT224973 NZ_CP120671:c3118065-3117691 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6I0CP94