Detailed information of TA system TA022497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2429069..2429696
• Replicon: chromosome
• Accession: NC_009922
• Organism: Alkaliphilus oremlandii OhILAs

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A8MJ24
• Locus tag: CLOS_RS11825
• Protein ID: WP_012160113.1
• Coordinates: 2429069..2429419 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A8MJ25
• Locus tag: CLOS_RS11830
• Protein ID: WP_012160114.1
• Coordinates: 2429421..2429696 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLOS_RS11805	2424372..2425100	-	729	WP_012160109.1	WecB/TagA/CpsF family glycosyltransferase	-
CLOS_RS11810	2425097..2426176	-	1080	WP_012160110.1	polysaccharide pyruvyl transferase CsaB	-
CLOS_RS11815	2426176..2428338	-	2163	WP_012160111.1	hypothetical protein	-
CLOS_RS11820	2428417..2428905	-	489	WP_012160112.1	hypothetical protein	-
CLOS_RS11825	2429069..2429419	-	351	WP_012160113.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CLOS_RS11830	2429421..2429696	-	276	WP_012160114.1	ribbon-helix-helix protein, CopG family	Antitoxin
CLOS_RS11835	2429793..2430965	-	1173	WP_012160115.1	alanine racemase	-
CLOS_RS11840	2430996..2431364	-	369	WP_012160116.1	holo-ACP synthase	-
CLOS_RS11845	2431558..2432172	-	615	WP_012160117.1	hypothetical protein	-
CLOS_RS11850	2432346..2433353	+	1008	WP_012160118.1	hypothetical protein	-
CLOS_RS11855	2433502..2433939	-	438	WP_012160119.1	hypothetical protein	-
CLOS_RS11860	2433956..2434315	-	360	WP_012160120.1	hypothetical protein	-
CLOS_RS11865	2434296..2434490	-	195	WP_041719330.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12893.01 Da
• Isoelectric Point: 5.7299

>T22497 WP_012160113.1 NC_009922:c2429419-2429069 [Alkaliphilus oremlandii OhILAs]
VIIKRGDIFYADLSPVIGSEQGGVRPVLIIQNDIGNRYSPTIIVVAITSQINKAKLPTHVEIKSSDYGLPKDSVLLLEQI
RTIDKRRLEEKVGHVTDDIMEKVNEALLISLGLIEF

Nucleotide

• Length: 351 bp

>T22497 NC_009922:c2429419-2429069 [Alkaliphilus oremlandii OhILAs]
GTGATTATAAAAAGAGGAGATATCTTTTATGCGGATCTAAGCCCTGTTATCGGCTCGGAGCAAGGGGGAGTAAGGCCTGT
ATTAATCATACAGAATGATATAGGAAACCGATATAGTCCTACCATTATCGTAGTTGCTATTACTTCGCAAATCAATAAAG
CCAAGCTTCCAACCCACGTTGAAATTAAATCCTCTGATTATGGATTACCAAAGGATTCAGTTTTGTTACTTGAGCAAATT
CGAACCATTGATAAAAGGCGTTTGGAGGAAAAAGTCGGCCATGTAACAGATGATATTATGGAGAAAGTAAATGAGGCCTT
ATTAATTAGTCTCGGTTTAATCGAATTCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10623.31 Da
• Isoelectric Point: 4.5826

>AT22497 WP_012160114.1 NC_009922:c2429696-2429421 [Alkaliphilus oremlandii OhILAs]
MADSKRIMISLPDSLLQEVDNIVSMEKTNRSEFIREAMKLYIRERNKMELREQMKRGYQEMGVINLALAELGLSLDMSSL
ETYEARMAECE

Nucleotide

• Length: 276 bp

>AT22497 NC_009922:c2429696-2429421 [Alkaliphilus oremlandii OhILAs]
ATGGCCGATTCAAAAAGAATTATGATAAGCCTTCCTGATAGTCTTTTACAAGAGGTAGATAATATTGTTTCTATGGAAAA
GACAAATAGAAGCGAGTTTATTCGTGAAGCAATGAAACTATACATTCGGGAGAGAAATAAAATGGAACTGAGAGAGCAGA
TGAAGAGAGGCTATCAAGAGATGGGAGTAATCAATCTGGCATTGGCCGAGCTTGGACTAAGTTTAGATATGTCTTCGTTA
GAAACCTACGAGGCGAGAATGGCGGAGTGTGAGTAA

Structures

Toxin

• AlphaFold DB: A8MJ24

Antitoxin

• AlphaFold DB: A8MJ25