Detailed information of TA system TA224968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1715038..1715654
• Replicon: chromosome
• Accession: NZ_CP087167
• Organism: Pseudomonas atacamensis strain B21-050

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY49_RS07580
• Protein ID: WP_076566640.1
• Coordinates: 1715038..1715250 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY49_RS07585
• Protein ID: WP_130901292.1
• Coordinates: 1715250..1715654 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY49_RS07550 (LOY49_07530)	1711140..1711676	+	537	WP_024012128.1	DsbE family thiol:disulfide interchange protein	-
LOY49_RS07555 (LOY49_07535)	1711673..1712143	+	471	WP_110601105.1	cytochrome c-type biogenesis protein CcmH	-
LOY49_RS07560 (LOY49_07540)	1712140..1713339	+	1200	WP_258605561.1	c-type cytochrome biogenesis protein CcmI	-
LOY49_RS07565 (LOY49_07545)	1713365..1713769	+	405	WP_016771039.1	hypothetical protein	-
LOY49_RS07570 (LOY49_07550)	1713815..1714390	-	576	WP_258605563.1	PIN domain-containing protein	-
LOY49_RS07575 (LOY49_07555)	1714387..1714854	-	468	WP_258605565.1	helix-turn-helix domain-containing protein	-
LOY49_RS07580 (LOY49_07560)	1715038..1715250	+	213	WP_076566640.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY49_RS07585 (LOY49_07565)	1715250..1715654	+	405	WP_130901292.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY49_RS07590 (LOY49_07570)	1715835..1717022	-	1188	WP_024012134.1	MFS transporter	-
LOY49_RS07595 (LOY49_07575)	1717189..1718187	-	999	WP_110601117.1	sulfate ABC transporter substrate-binding protein	-
LOY49_RS07600 (LOY49_07580)	1718292..1719116	-	825	WP_016771032.1	ion transporter	-
LOY49_RS07605 (LOY49_07585)	1719138..1720052	-	915	WP_258605570.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7934.09 Da
• Isoelectric Point: 10.8102

>T224968 WP_076566640.1 NZ_CP087167:1715038-1715250 [Pseudomonas atacamensis]
VQSRLLMKELEEAGWTLDRVAGSHHIFTHRYNPNTIAVPHPKKDLPLGTVKSIRRRAGLFSPPTRHEGDP

Nucleotide

• Length: 213 bp

>T224968 NZ_CP120671:c1628859-1628548 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14804.86 Da
• Isoelectric Point: 4.5789

>AT224968 WP_130901292.1 NZ_CP087167:1715250-1715654 [Pseudomonas atacamensis]
MQYPICIEWGDENTAIGIQIPDIPGAVTVGDSFEEAYNAAVEVAHLMLQEIAAAGQAIPMPTSAAAHRNHPDFAEMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT224968 NZ_CP120671:c1628551-1628105 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG