Detailed information of TA system TA224966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 878113..878702
• Replicon: chromosome
• Accession: NZ_CP087167
• Organism: Pseudomonas atacamensis strain B21-050

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY49_RS03715
• Protein ID: WP_217879323.1
• Coordinates: 878451..878702 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY49_RS03710
• Protein ID: WP_056782875.1
• Coordinates: 878113..878454 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY49_RS03685 (LOY49_03675)	873816..874217	+	402	WP_016771753.1	30S ribosome-binding factor RbfA	-
LOY49_RS03690 (LOY49_03680)	874222..875139	+	918	WP_024011571.1	tRNA pseudouridine(55) synthase TruB	-
LOY49_RS03695 (LOY49_03685)	875283..875552	+	270	WP_016771751.1	30S ribosomal protein S15	-
LOY49_RS03700 (LOY49_03690)	875757..877862	+	2106	WP_024011572.1	polyribonucleotide nucleotidyltransferase	-
LOY49_RS03710 (LOY49_03700)	878113..878454	-	342	WP_056782875.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY49_RS03715 (LOY49_03705)	878451..878702	-	252	WP_217879323.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY49_RS03720 (LOY49_03710)	879058..879357	+	300	WP_258604893.1	DUF6388 family protein	-
LOY49_RS03725 (LOY49_03715)	879445..880293	-	849	WP_016771747.1	carboxylating nicotinate-nucleotide diphosphorylase	-
LOY49_RS03730 (LOY49_03720)	880561..882738	+	2178	WP_258604895.1	DUF1631 domain-containing protein	-
LOY49_RS03735 (LOY49_03725)	882803..883363	+	561	WP_122699435.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9183.59 Da
• Isoelectric Point: 10.3167

>T224966 WP_217879323.1 NZ_CP087167:c878702-878451 [Pseudomonas atacamensis]
MSKTEKLLAKLINGHSAFTWSELVTLLGRLGYSQLEGSGSRIKFDNGDPNALINLHKPHPGNEIKTYARRQIIEQLKTGG
LIP

Nucleotide

• Length: 252 bp

>T224966 NZ_CP120671:c1122338-1121970 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTTGCTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12593.23 Da
• Isoelectric Point: 6.3189

>AT224966 WP_056782875.1 NZ_CP087167:c878454-878113 [Pseudomonas atacamensis]
MSSQLHYKGYVGSIEASTEDKCLFGKLLFIRALVNYEGQTVAELEAAFREAVDDYLQTCQQLGQKPEIPCKGSFNVRIGH
DLHLAAALAATRQNVSLNDLTRQALNEYLQHHA

Nucleotide

• Length: 342 bp

>AT224966 NZ_CP120671:c1122556-1122335 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA