Detailed information of TA system TA224965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 474781..475412
• Replicon: chromosome
• Accession: NZ_CP087167
• Organism: Pseudomonas atacamensis strain B21-050

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1N7CQ04
• Locus tag: LOY49_RS02090
• Protein ID: WP_041477600.1
• Coordinates: 475050..475412 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LOY49_RS02085
• Protein ID: WP_258604589.1
• Coordinates: 474781..475020 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY49_RS02050 (LOY49_02045)	470280..470825	-	546	WP_016772027.1	membrane protein	-
LOY49_RS02055 (LOY49_02050)	470822..471076	-	255	WP_016772026.1	acyl carrier protein	-
LOY49_RS02060 (LOY49_02055)	471086..471361	-	276	WP_122699832.1	phosphopantetheine-binding protein	-
LOY49_RS02065 (LOY49_02060)	471339..472151	-	813	WP_258604587.1	lysophospholipid acyltransferase family protein	-
LOY49_RS02070 (LOY49_02065)	472127..472852	-	726	WP_016772023.1	beta-ketoacyl synthase chain length factor	-
LOY49_RS02075 (LOY49_02070)	473209..473979	+	771	WP_016772022.1	ParA family protein	-
LOY49_RS02080 (LOY49_02075)	474110..474544	-	435	WP_122611051.1	thioredoxin TrxC	-
LOY49_RS02085 (LOY49_02080)	474781..475020	+	240	WP_258604589.1	PbsX family transcriptional regulator	Antitoxin
LOY49_RS02090 (LOY49_02085)	475050..475412	+	363	WP_041477600.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOY49_RS02095 (LOY49_02090)	475418..476881	-	1464	WP_258604591.1	YdiU family protein	-
LOY49_RS02100 (LOY49_02095)	476930..480283	-	3354	WP_258604593.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13337.24 Da
• Isoelectric Point: 7.1674

>T224965 WP_041477600.1 NZ_CP087167:475050-475412 [Pseudomonas atacamensis]
MVRRQPPQRGDVYWIDPNPVAGREMMNRHRFVVITPREINALGVSMTVPVTSGGNFSRNMGLAVAITGHETNGVAVCNQV
RSFDIEQRVRDGTAKFIERLDDVTMSDIVDRVISAIDPLS

Nucleotide

• Length: 363 bp

>T224965 NZ_CP120671:c602875-602462 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8491.88 Da
• Isoelectric Point: 7.0112

>AT224965 WP_258604589.1 NZ_CP087167:474781-475020 [Pseudomonas atacamensis]
MSVTSKIRRQGGAAVITIPPTLLKLLHLEVGAQLELNVVDGELIARPAVAAARKRYSLSELLEGAEELQKLNAQTSETL

Nucleotide

• Length: 240 bp

>AT224965 NZ_CP120671:c603122-602856 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1N7CQ04