Detailed information of TA system TA022496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4673505..4675105
• Replicon: chromosome
• Accession: NC_009901
• Organism: Shewanella pealeana ATCC 700345

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A8H9B9
• Locus tag: SPEA_RS20205
• Protein ID: WP_012157038.1
• Coordinates: 4673505..4674824 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: SPEA_RS20210
• Protein ID: WP_012157039.1
• Coordinates: 4674824..4675105 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPEA_RS20185	4669611..4670270	+	660	WP_012157034.1	GTP cyclohydrolase I FolE	-
SPEA_RS20190	4670794..4671435	-	642	WP_012157035.1	orotate phosphoribosyltransferase	-
SPEA_RS20195	4671555..4672268	-	714	WP_012157036.1	ribonuclease PH	-
SPEA_RS20200	4672541..4673404	+	864	WP_012157037.1	YicC family protein	-
SPEA_RS20205	4673505..4674824	-	1320	WP_012157038.1	type II toxin-antitoxin system HipA family toxin	Toxin
SPEA_RS20210	4674824..4675105	-	282	WP_012157039.1	helix-turn-helix domain-containing protein	Antitoxin
SPEA_RS20215	4675464..4675730	+	267	Protein_3844	DUF1732 domain-containing protein	-
SPEA_RS20220	4675864..4677105	-	1242	WP_150102326.1	restriction endonuclease	-
SPEA_RS20225	4677747..4678586	+	840	WP_012157042.1	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	-
SPEA_RS20230	4678747..4680015	+	1269	WP_012157043.1	NupC/NupG family nucleoside CNT transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49077.52 Da
• Isoelectric Point: 6.5066

>T22496 WP_012157038.1 NC_009901:c4674824-4673505 [Shewanella pealeana ATCC 700345]
MAVLDVYMNGYLVGEFTKSTTGSHLFKYAAQWLDTPGSRPISLSMPLRSQAYKGDEVYNFFDNLLPDNMEVRNRIVARHN
AQSTQAFDLLAKVGQDSVGALQLVPHEQQPFEVKKIEAKPLSDEALEKVLKGYQSDIPLGMLSDEDDFRISIAGAQEKTA
LLNLDGRWQLPIKATPTTHIIKLPIGEIQSHSYTIDMSDSVENEYLCMMIVREFGLDVPDCSIIKVGDNKALAVARFDRK
LSSDGQWIMRLPQEDFCQTLNIPSAKKYESHGGPGVEHIIPYLLGSMNSEQDRYNFMKSQVLFWLLAATDGHAKNFSLFI
EVGGGYRLTPFYDIISMYPVIGGKGLNIRDAKLAMGLKATKGKKYQIVKIFPKHFIETAKAVGFDLATMAAILREVADSV
DNVIMKVKTQLPEDFPVHIRDSILDGLQDRAKRLMAGAY

Nucleotide

• Length: 1320 bp

>T22496 NC_009901:c4674824-4673505 [Shewanella pealeana ATCC 700345]
ATGGCAGTTCTGGATGTTTATATGAATGGCTATTTGGTCGGTGAGTTTACTAAATCGACCACAGGCTCTCATCTGTTTAA
GTATGCAGCCCAATGGCTAGATACGCCTGGCAGTCGTCCTATTTCACTGTCTATGCCATTGCGTTCGCAAGCGTATAAAG
GCGATGAGGTTTATAACTTCTTTGATAATTTACTGCCAGATAACATGGAGGTGAGAAACAGGATTGTTGCTCGTCACAAT
GCGCAATCGACACAAGCGTTCGATCTACTCGCTAAAGTCGGTCAAGACAGTGTTGGTGCACTGCAGTTAGTACCCCATGA
GCAGCAACCCTTTGAGGTTAAAAAGATTGAAGCTAAACCTTTATCTGATGAGGCATTGGAAAAAGTGCTCAAAGGTTATC
AGTCAGATATTCCATTAGGCATGCTAAGCGATGAAGATGATTTTCGAATATCGATTGCAGGTGCCCAAGAGAAGACCGCC
TTACTTAATTTAGATGGCCGATGGCAGCTTCCAATTAAAGCTACACCGACTACACATATCATTAAGTTGCCTATCGGTGA
GATACAGAGTCATTCATACACTATTGATATGTCTGACAGCGTTGAAAACGAATATCTGTGTATGATGATAGTAAGGGAGT
TTGGCTTAGATGTACCAGATTGCTCAATAATTAAAGTTGGTGATAATAAGGCGCTGGCAGTAGCACGTTTTGATCGTAAA
TTATCTAGCGATGGCCAATGGATTATGCGATTGCCACAAGAGGATTTCTGCCAAACATTAAATATTCCATCGGCTAAAAA
ATATGAAAGTCACGGCGGGCCGGGTGTTGAGCATATCATACCTTATTTACTGGGCTCTATGAACTCAGAGCAAGATAGGT
ATAACTTTATGAAGTCGCAAGTGCTGTTCTGGCTTTTAGCGGCAACAGACGGCCATGCTAAAAACTTCTCGCTATTTATT
GAGGTTGGCGGCGGGTATCGATTAACTCCCTTTTACGACATTATATCCATGTATCCAGTAATTGGTGGCAAGGGCCTAAA
TATCCGTGATGCCAAGCTTGCTATGGGGCTCAAAGCTACCAAAGGTAAGAAGTATCAGATAGTAAAGATTTTCCCTAAAC
ACTTTATCGAAACTGCGAAAGCGGTTGGCTTTGATCTCGCTACTATGGCAGCCATTTTGCGAGAGGTTGCTGATTCAGTA
GATAACGTGATTATGAAGGTTAAGACTCAGTTGCCCGAAGACTTTCCTGTACATATACGAGATTCGATTTTGGATGGTTT
GCAGGATAGAGCAAAGCGCCTGATGGCGGGGGCGTACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10633.00 Da
• Isoelectric Point: 6.3713

>AT22496 WP_012157039.1 NC_009901:c4675105-4674824 [Shewanella pealeana ATCC 700345]
MKVTNSKQLSAYLKDARLNMKLSQSKVGSKVGIRQDTVSSFEQNPDSTKLETLFKILSALNLELDIKVRNSELAERLNDD
KSTASEQEWKEEW

Nucleotide

• Length: 282 bp

>AT22496 NC_009901:c4675105-4674824 [Shewanella pealeana ATCC 700345]
ATGAAGGTGACGAACTCAAAGCAGCTTAGCGCATATCTCAAAGATGCACGATTGAATATGAAGCTCTCGCAAAGCAAGGT
AGGTAGTAAAGTCGGCATTCGTCAGGATACAGTTTCAAGCTTTGAACAGAACCCTGACTCCACCAAGCTAGAGACGCTGT
TTAAAATTCTATCAGCACTGAACCTTGAGCTGGATATAAAGGTTAGGAATTCAGAACTAGCGGAACGGCTGAATGATGAT
AAAAGCACCGCCTCCGAGCAAGAGTGGAAGGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A8H9B9