Detailed information of TA system TA224959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1736590..1737206
• Replicon: chromosome
• Accession: NZ_CP087166
• Organism: Pseudomonas sp. B21-051

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W6VL41
• Locus tag: LOY52_RS07850
• Protein ID: WP_007961665.1
• Coordinates: 1736590..1736802 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W6W5D4
• Locus tag: LOY52_RS07855
• Protein ID: WP_007961664.1
• Coordinates: 1736802..1737206 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY52_RS07825 (LOY52_07815)	1732714..1733250	+	537	WP_258678977.1	DsbE family thiol:disulfide interchange protein	-
LOY52_RS07830 (LOY52_07820)	1733247..1733717	+	471	WP_007961669.1	cytochrome c-type biogenesis protein CcmH	-
LOY52_RS07835 (LOY52_07825)	1733714..1734916	+	1203	WP_258678978.1	c-type cytochrome biogenesis protein CcmI	-
LOY52_RS07840 (LOY52_07830)	1734941..1735345	+	405	WP_007961667.1	hypothetical protein	-
LOY52_RS07845 (LOY52_07835)	1735433..1736320	-	888	WP_258678979.1	LysR family transcriptional regulator	-
LOY52_RS07850 (LOY52_07840)	1736590..1736802	+	213	WP_007961665.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY52_RS07855 (LOY52_07845)	1736802..1737206	+	405	WP_007961664.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY52_RS07860 (LOY52_07850)	1737261..1738448	-	1188	WP_258678980.1	MFS transporter	-
LOY52_RS07865 (LOY52_07855)	1738692..1739312	+	621	WP_258678981.1	DUF5666 domain-containing protein	-
LOY52_RS07870 (LOY52_07860)	1739474..1740469	-	996	WP_258678982.1	sulfate ABC transporter substrate-binding protein	-
LOY52_RS07875 (LOY52_07865)	1740649..1741473	-	825	WP_258678983.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7964.14 Da
• Isoelectric Point: 10.5308

>T224959 WP_007961665.1 NZ_CP087166:1736590-1736802 [Pseudomonas sp. B21-051]
VQSRLLIKELEEAGWTLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVKSIRRRAGLYHPPASNKGDP

Nucleotide

• Length: 213 bp

>T224959 NZ_CP120641:533177-533527 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14836.80 Da
• Isoelectric Point: 4.2870

>AT224959 WP_007961664.1 NZ_CP087166:1736802-1737206 [Pseudomonas sp. B21-051]
MQYPICIEWGDDNTAIGIQIPDIPGAVTAGDTFEDAYNAAVEVAHLMLQEMAADGESIPMPTSAAAHRNNPEFADMGWGM
LELDISPYMGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT224959 NZ_CP120641:532890-533171 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: W6VL41

Antitoxin

• AlphaFold DB: W6W5D4