Detailed information of TA system TA224958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1483988..1484611
• Replicon: chromosome
• Accession: NZ_CP087166
• Organism: Pseudomonas sp. B21-051

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W6VV48
• Locus tag: LOY52_RS06635
• Protein ID: WP_007963251.1
• Coordinates: 1484432..1484611 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY52_RS06630
• Protein ID: WP_247267806.1
• Coordinates: 1483988..1484395 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY52_RS06600 (LOY52_06595)	1479301..1480650	+	1350	WP_258678831.1	diguanylate cyclase	-
LOY52_RS06605 (LOY52_06600)	1480769..1480927	+	159	WP_003222499.1	KGG domain-containing protein	-
LOY52_RS06610 (LOY52_06605)	1481003..1481506	+	504	WP_027614439.1	DUF4142 domain-containing protein	-
LOY52_RS06615 (LOY52_06610)	1481608..1482018	-	411	WP_047598058.1	low affinity iron permease family protein	-
LOY52_RS06620 (LOY52_06615)	1482206..1482976	+	771	WP_258678833.1	trans-aconitate 2-methyltransferase	-
LOY52_RS06625 (LOY52_06620)	1483103..1483831	-	729	WP_258678835.1	class I SAM-dependent methyltransferase	-
LOY52_RS06630 (LOY52_06625)	1483988..1484395	-	408	WP_247267806.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY52_RS06635 (LOY52_06630)	1484432..1484611	-	180	WP_007963251.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY52_RS06645 (LOY52_06640)	1485109..1485993	-	885	WP_258678838.1	alpha/beta hydrolase	-
LOY52_RS06650 (LOY52_06645)	1486310..1487002	-	693	WP_258678840.1	pseudouridine synthase	-
LOY52_RS06655 (LOY52_06650)	1487036..1487266	-	231	WP_258678841.1	cysteine-rich CWC family protein	-
LOY52_RS06660 (LOY52_06655)	1487641..1487913	+	273	WP_258678843.1	hypothetical protein	-
LOY52_RS06665 (LOY52_06660)	1488082..1488480	+	399	WP_007963255.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6645.00 Da
• Isoelectric Point: 11.2641

>T224958 WP_007963251.1 NZ_CP087166:c1484611-1484432 [Pseudomonas sp. B21-051]
VNSRFLIGQLVADGWYLVRVKGSHHHFKHPIKPGLVTVPHPKKDLLKKTAISILKQALL

Nucleotide

• Length: 180 bp

>T224958 NZ_CP120639:936512-937009 [Rhizobium sp. 32-5/1]
GTGAGCATAACTGCGCCTCCTCCTTTGGCTGATCATCATGAGTTGGCCGAGTTCGATTCTGGCGTTTCTGAGCTGGACGA
TTGGCTTTGTCGACGAGCCCGTACCAACCAGGTAAGTGGCGCGTCACGTACCTTTGTTGTTTGCGAAGGAAGTCGTGTCA
TTGCCTACTACGCGCTTGCCTCAGGCGCGGTGAAGCCGCCAGAAGCACCCGGTCGTCTTAGGCGCAACATGCCGGATCCG
ATCCGAGTCGCCGTGCTAGGCCGGCTAGCTATTGACCGGTCATATCAAGGCCGAGGTATCGGTCGCGCGCTGGTCCGCGA
TGTGGGACTGCGCTTGCTCAATGCCGCTGAAATACTTGGCATCCGTGGCGTGCTCGTGCATGCAATTTCAGATGAAGCAC
GAGCCTTCTATGAGGCTGTCGGCTTCCTTCCTTCTCCGTCAGACCCAATGATGCTGATGATAGGACTAGGAGACCTCAAT
AGTGCGCTTGCCGACTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14511.48 Da
• Isoelectric Point: 4.4893

>AT224958 WP_247267806.1 NZ_CP087166:c1484395-1483988 [Pseudomonas sp. B21-051]
MLYPIAISIGDEEHAWGVEVPDIPGCFSAGDDLDDAVAMAREAIEGHFEILAEDGSPIPPASKLGVHVVNPQYAGCAWAV
VDIDVTKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT224958 NZ_CP120639:936219-936515 [Rhizobium sp. 32-5/1]
ATGTCGACGTCACACACGAGTAAGAGCGTGGACGCAAGCGTTCCACTAAATATGCGTATTAAGCCGGCGACGCGCAATTT
GATTGACCGTGCGGCTGAGTTGCTTGGCAAGACTCGCACAGATTTCATGTTGGAGGCGTCTGAACGTCGAGCTGAGGAAG
TGCTGCTCGATCGTACGATTTTCACCGTGAGTCCCGTAGTCTATGCCGAGTACCTTGCTCGCCTTGATGCGCCCGCGCAG
AGCAATGAGCGTTTGAAACGCACGATGTCCACCAAAGCGCCGTGGGACAAGGCGTGA

Structures

Toxin

• AlphaFold DB: W6VV48