Detailed information of TA system TA224955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5067543..5068192
• Replicon: chromosome
• Accession: NZ_CP087165
• Organism: Pseudomonas sichuanensis strain B21-052

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LOY46_RS23565
• Protein ID: WP_125465620.1
• Coordinates: 5068010..5068192 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOY46_RS23560
• Protein ID: WP_125465619.1
• Coordinates: 5067543..5067953 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY46_RS23535 (LOY46_23505)	5063548..5064453	-	906	WP_125465614.1	LysR family transcriptional regulator	-
LOY46_RS23540 (LOY46_23510)	5064591..5065181	-	591	WP_125860468.1	NAD(P)H-dependent oxidoreductase	-
LOY46_RS23545 (LOY46_23515)	5065359..5065673	+	315	WP_125465616.1	putative quinol monooxygenase	-
LOY46_RS23550 (LOY46_23520)	5065663..5066427	-	765	WP_125860472.1	sulfite exporter TauE/SafE family protein	-
LOY46_RS23555 (LOY46_23525)	5066505..5067389	+	885	WP_258705143.1	LysR family transcriptional regulator	-
LOY46_RS23560 (LOY46_23530)	5067543..5067953	-	411	WP_125465619.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOY46_RS23565 (LOY46_23535)	5068010..5068192	-	183	WP_125465620.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOY46_RS23570 (LOY46_23540)	5068406..5069011	+	606	WP_258705144.1	CDP-alcohol phosphatidyltransferase family protein	-
LOY46_RS23575 (LOY46_23545)	5069042..5070796	+	1755	WP_258705145.1	bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase	-
LOY46_RS23580 (LOY46_23550)	5070796..5072199	+	1404	WP_258705146.1	phosphatase PAP2/dual specificity phosphatase family protein	-
LOY46_RS23585 (LOY46_23555)	5072196..5072615	+	420	WP_258705147.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6942.97 Da
• Isoelectric Point: 10.7251

>T224955 WP_125465620.1 NZ_CP087165:c5068192-5068010 [Pseudomonas sichuanensis]
MRSRDVIQLIEADGWYEVEVKGSHHQFRHPYKRGRVTVPHPKSDLPRGTVHSILKQAGLK

Nucleotide

• Length: 183 bp

>T224955 NZ_CP120639:c252232-251846 [Rhizobium sp. 32-5/1]
ATGGTGATCGACACGTCCGCGATCGTCGCGATCTTCTTCAATGAGCCGGACGCCGCGAACTATCGCGAACGCATCGCCAA
CGATCCTATCAGGTTCATTTCGGCGGCAACGCTCGTCGAGGCCTCGATGGTGATCGAGGGGCGTTTTGGCGAAGCCGGCG
GAGCAGAGCTCGATCTCTGGGTGCACAAGGCAAACGTGGAAGTCGTTGCGGTGACCGCCGAGCACGCCGACCAGGCTCGC
CGGGCCTGGCGTCGCTATGGCAAGGGCCGTCATCCCGCTTCACTGAATTACGGGGATTGCTTCTCCTACGCGCTTGCCAC
GCTGGCCGGCGAGCCGCTTCTCTTCAAGGGTAACGACTTCAAACAGACGAATATCCGAGCGGCATAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14723.62 Da
• Isoelectric Point: 4.6138

>AT224955 WP_125465619.1 NZ_CP087165:c5067953-5067543 [Pseudomonas sichuanensis]
MKFPVVLHKDEGSDYGVIVPDVPGCFSAGATVSQALENVQEALALHFEGLVADGEALPQAEEVDVHVANPDYAGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELAVAFR

Nucleotide

• Length: 411 bp

>AT224955 NZ_CP120639:c252486-252232 [Rhizobium sp. 32-5/1]
ATGGCGCTAAGTATCAAAGATCCCGAGACGGAGAAACTCGCGCGTGCACTCGCAGCACGCACCGGTGAAACCATCACCAT
CGCGACGCGGCGCGCGCTGGAAGAGCGCCTCAAGCGAATGGGATCAAACGCGAAGAAAGATGCCCTCATGCAAGACATGG
AGGCGATGCAGCGGCGTTTAATCGCTTTGCCTATTCTCGATGACCGCAGTGCTGATGACATCATCGGGTACGACGAAAAC
GGTCTTCCAAACTGA