Detailed information of TA system TA224953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3995056..3996095
• Replicon: chromosome
• Accession: NZ_CP087165
• Organism: Pseudomonas sichuanensis strain B21-052

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LOY46_RS18745
• Protein ID: WP_258704795.1
• Coordinates: 3995520..3996095 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LOY46_RS18740
• Protein ID: WP_258704794.1
• Coordinates: 3995056..3995523 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY46_RS18720 (LOY46_18700)	3990674..3991693	+	1020	WP_125464851.1	ABC transporter permease	-
LOY46_RS18725 (LOY46_18705)	3991695..3993305	+	1611	WP_258704791.1	ABC transporter ATP-binding protein	-
LOY46_RS18730 (LOY46_18710)	3994060..3994308	+	249	WP_258704792.1	hypothetical protein	-
LOY46_RS18735 (LOY46_18715)	3994463..3994732	+	270	WP_258704793.1	DUF3077 domain-containing protein	-
LOY46_RS18740 (LOY46_18720)	3995056..3995523	+	468	WP_258704794.1	helix-turn-helix domain-containing protein	Antitoxin
LOY46_RS18745 (LOY46_18725)	3995520..3996095	+	576	WP_258704795.1	PIN domain-containing protein	Toxin
LOY46_RS18750 (LOY46_18730)	3996309..3997361	+	1053	WP_258704796.1	DUF2235 domain-containing protein	-
LOY46_RS18755 (LOY46_18735)	3997380..3997745	-	366	WP_258704797.1	hypothetical protein	-
LOY46_RS18760 (LOY46_18740)	3997742..3998710	-	969	WP_258704798.1	LysR family transcriptional regulator	-
LOY46_RS18765 (LOY46_18745)	3998996..4000231	+	1236	WP_258704799.1	diaminopimelate decarboxylase	-
LOY46_RS18770 (LOY46_18750)	4000361..4001059	-	699	WP_153786626.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21601.75 Da
• Isoelectric Point: 4.7714

>T224953 WP_258704795.1 NZ_CP087165:3995520-3996095 [Pseudomonas sichuanensis]
MKHSSFTALYDANVLYPAPLRDLLMNLALTGVYRARWLARIHDEWKRNLLINRPDLDRTQLDRTSGLMDEAVPDALVSDY
EPLMGGLDLPDEDDRHVLAAAIKCNASVIVTFNLKDFPDTALGSFDIEALHPDDFIADLRDLDKAAVLEAAQRQRQSLKN
PALSVQAYLDKLLQQKLPESVKLLSEFRFLL

Nucleotide

• Length: 576 bp

>T224953 NZ_CP120638:3646151-3646420 [Rhizobium sp. 32-5/1]
TTGGTCTGGACGATTGACATAACCAACAATGCTGAAAAGGCATTGCGGAAGATGGGCTCTTCCGATGTGCGTCGCCTCCG
CGACTTTCTTTTTGGCCGGGTTGCGCTTCTCGACGATCCTCGCCAGATCGGCCAAGCCTTGCAGGGCAATGAACTCGGTC
ATCTCTGGCGTTACCGTGTCGGGGACTTTCGCGTTCTCTGTGAAATCCGCGATAACCGCCTTGTTATTCTTGTGGTCGAA
ATCGGTCATCGTCGCGAAGTCTACAGATGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17203.67 Da
• Isoelectric Point: 6.3371

>AT224953 WP_258704794.1 NZ_CP087165:3995056-3995523 [Pseudomonas sichuanensis]
MTTTDLSRNMLPDDNEIAVAVESSRTLAAFLSTAAHTQRIELLNDANQRQVVEVPTFALRLFGEILSELALGNAVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEGLMPHTKTGRHRRVKFADLVAYKNKRDAISRTAMDELAAQAQDLRMGYE

Nucleotide

• Length: 468 bp

>AT224953 NZ_CP120638:3645934-3646167 [Rhizobium sp. 32-5/1]
ATGACACGACAGACTGCGATACGTCTCCCCGATGAGACATATGAACGGCTACAAGTGCTGGCGACGCGGACCGGCAGGAC
CGCGACCTATTATATCCGGGAAGCGATCGAACAGCATCTTGAAGACCTCGAGGATATCTATCTGGCGGAGCAGGTTCTGG
AGAAGCTGGCCCGCGGTGAAATCACCACACGCCCTCTAGCGGAAGTGATGGCCGAGCTTGGTCTGGACGATTGA