Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tad-ata/COG5606-COG4679 |
| Location | 339432..340125 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP087161 | ||
| Organism | Pseudomonas sp. CIP-10 | ||
Toxin (Protein)
| Gene name | tad | Uniprot ID | N2IHR9 |
| Locus tag | LMH93_RS28805 | Protein ID | WP_003151133.1 |
| Coordinates | 339432..339809 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | ata | Uniprot ID | N2IIN5 |
| Locus tag | LMH93_RS28810 | Protein ID | WP_001172026.1 |
| Coordinates | 339790..340125 (+) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH93_RS28780 (LMH93_28780) | 335222..336106 | + | 885 | WP_003465017.1 | LysR family transcriptional regulator | - |
| LMH93_RS28785 (LMH93_28785) | 336290..336890 | - | 601 | Protein_343 | uracil-DNA glycosylase family protein | - |
| LMH93_RS28790 (LMH93_28790) | 336964..337512 | - | 549 | Protein_344 | cytochrome P460 family protein | - |
| LMH93_RS28795 (LMH93_28795) | 337540..338073 | - | 534 | WP_020309197.1 | carboxymuconolactone decarboxylase family protein | - |
| LMH93_RS28800 (LMH93_28800) | 338191..339033 | + | 843 | WP_049275859.1 | AraC family transcriptional regulator | - |
| LMH93_RS28805 (LMH93_28805) | 339432..339809 | + | 378 | WP_003151133.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LMH93_RS28810 (LMH93_28810) | 339790..340125 | + | 336 | WP_001172026.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| LMH93_RS28815 (LMH93_28815) | 340140..340475 | + | 336 | WP_000741275.1 | hypothetical protein | - |
| LMH93_RS28820 (LMH93_28820) | 340499..340825 | + | 327 | WP_000091614.1 | hypothetical protein | - |
| LMH93_RS28825 (LMH93_28825) | 340822..341202 | + | 381 | WP_001054412.1 | hypothetical protein | - |
| LMH93_RS28830 (LMH93_28830) | 341359..341730 | + | 372 | WP_230065443.1 | DUF3391 domain-containing protein | - |
| LMH93_RS28835 (LMH93_28835) | 341822..342691 | + | 870 | WP_223198360.1 | HD-GYP domain-containing protein | - |
| LMH93_RS28840 (LMH93_28840) | 343465..343872 | - | 408 | WP_023383747.1 | hypothetical protein | - |
| LMH93_RS28845 (LMH93_28845) | 343909..344601 | - | 693 | WP_023383746.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..395258 | 395258 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13705.78 Da Isoelectric Point: 9.4693
>T224947 WP_003151133.1 NZ_CP087161:339432-339809 [Pseudomonas sp. CIP-10]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH
Download Length: 378 bp
>T224947 NZ_CP120637:490252-490602 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 112 a.a. Molecular weight: 12201.39 Da Isoelectric Point: 10.6058
>AT224947 WP_001172026.1 NZ_CP087161:339790-340125 [Pseudomonas sp. CIP-10]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA
Download Length: 336 bp
>AT224947 NZ_CP120637:489965-490246 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A024ELN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A024EKI7 |