Detailed information of TA system TA224944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1948193..1949232
• Replicon: chromosome
• Accession: NZ_CP087160
• Organism: Pseudomonas sp. CIP-10

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LMH93_RS08675
• Protein ID: WP_230065017.1
• Coordinates: 1948193..1948768 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A173HI04
• Locus tag: LMH93_RS08680
• Protein ID: WP_003251469.1
• Coordinates: 1948765..1949232 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH93_RS08665 (LMH93_08665)	1943810..1945045	+	1236	WP_230065016.1	NAD(P)/FAD-dependent oxidoreductase	-
LMH93_RS08670 (LMH93_08670)	1945049..1947979	-	2931	WP_102885721.1	aminotransferase	-
LMH93_RS08675 (LMH93_08675)	1948193..1948768	-	576	WP_230065017.1	PIN domain-containing protein	Toxin
LMH93_RS08680 (LMH93_08680)	1948765..1949232	-	468	WP_003251469.1	helix-turn-helix domain-containing protein	Antitoxin
LMH93_RS08685 (LMH93_08685)	1949377..1950984	-	1608	WP_003251468.1	ABC transporter ATP-binding protein	-
LMH93_RS08690 (LMH93_08690)	1950986..1952005	-	1020	WP_010954919.1	ABC transporter permease	-
LMH93_RS08695 (LMH93_08695)	1952007..1953080	-	1074	WP_003251466.1	microcin C ABC transporter permease YejB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21713.95 Da
• Isoelectric Point: 5.5710

>T224944 WP_230065017.1 NZ_CP087160:c1948768-1948193 [Pseudomonas sp. CIP-10]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDATVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFPKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHNVQQYLDRLLQQKLPETVKLLSAFKFLL

Nucleotide

• Length: 576 bp

>T224944 NZ_CP120633:4630671-4630973 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17229.71 Da
• Isoelectric Point: 6.6505

>AT224944 WP_003251469.1 NZ_CP087160:c1949232-1948765 [Pseudomonas sp. CIP-10]
MTTANLSRHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRTAMDALAAQAQELKMGYE

Nucleotide

• Length: 468 bp

>AT224944 NZ_CP120633:4631009-4631350 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A173HI04