Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5395352..5395977 Replicon chromosome
Accession NZ_CP087151
Organism Klebsiella pneumoniae strain KP14

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LOX93_RS26655 Protein ID WP_002882817.1
Coordinates 5395352..5395735 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LOX93_RS26660 Protein ID WP_004150355.1
Coordinates 5395735..5395977 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX93_RS26640 (LOX93_26640) 5392718..5393620 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LOX93_RS26645 (LOX93_26645) 5393617..5394252 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LOX93_RS26650 (LOX93_26650) 5394249..5395178 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LOX93_RS26655 (LOX93_26655) 5395352..5395735 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LOX93_RS26660 (LOX93_26660) 5395735..5395977 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LOX93_RS26665 (LOX93_26665) 5396182..5397099 + 918 WP_002882812.1 alpha/beta hydrolase -
LOX93_RS26670 (LOX93_26670) 5397113..5398054 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LOX93_RS26675 (LOX93_26675) 5398099..5398536 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LOX93_RS26680 (LOX93_26680) 5398533..5399393 - 861 WP_002882807.1 virulence factor BrkB family protein -
LOX93_RS26685 (LOX93_26685) 5399387..5399986 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T224932 WP_002882817.1 NZ_CP087151:c5395735-5395352 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T224932 NZ_CP120633:c2566079-2565765 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT224932 WP_004150355.1 NZ_CP087151:c5395977-5395735 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT224932 NZ_CP120633:c2565768-2565352 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References