Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4195429..4196048 Replicon chromosome
Accession NZ_CP087151
Organism Klebsiella pneumoniae strain KP14

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LOX93_RS20905 Protein ID WP_002892050.1
Coordinates 4195830..4196048 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LOX93_RS20900 Protein ID WP_002892066.1
Coordinates 4195429..4195803 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX93_RS20890 (LOX93_20890) 4190581..4191774 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LOX93_RS20895 (LOX93_20895) 4191797..4194943 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LOX93_RS20900 (LOX93_20900) 4195429..4195803 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LOX93_RS20905 (LOX93_20905) 4195830..4196048 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LOX93_RS20910 (LOX93_20910) 4196207..4196773 + 567 WP_002892030.1 maltose O-acetyltransferase -
LOX93_RS20915 (LOX93_20915) 4196745..4196885 - 141 WP_004147370.1 hypothetical protein -
LOX93_RS20920 (LOX93_20920) 4196906..4197376 + 471 WP_002892026.1 YlaC family protein -
LOX93_RS20925 (LOX93_20925) 4197351..4198802 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LOX93_RS20930 (LOX93_20930) 4198903..4199601 + 699 WP_002892021.1 GNAT family protein -
LOX93_RS20935 (LOX93_20935) 4199598..4199738 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LOX93_RS20940 (LOX93_20940) 4199738..4200001 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224928 WP_002892050.1 NZ_CP087151:4195830-4196048 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T224928 NZ_CP120633:c1316285-1316031 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT224928 WP_002892066.1 NZ_CP087151:4195429-4195803 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT224928 NZ_CP120633:c1316533-1316282 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References