Detailed information of TA system TA224918

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 875781..876435
• Replicon: chromosome
• Accession: NZ_CP087151
• Organism: Klebsiella pneumoniae strain KP14

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R4YHX2
• Locus tag: LOX93_RS04420
• Protein ID: WP_004144731.1
• Coordinates: 876028..876435 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: LOX93_RS04415
• Protein ID: WP_002916312.1
• Coordinates: 875781..876047 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX93_RS04390 (LOX93_04390)	870937..872370	-	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-
LOX93_RS04395 (LOX93_04395)	872489..873217	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
LOX93_RS04400 (LOX93_04400)	873267..873578	+	312	WP_002916319.1	N(4)-acetylcytidine aminohydrolase	-
LOX93_RS04405 (LOX93_04405)	873742..874401	+	660	WP_002916317.1	hemolysin III family protein	-
LOX93_RS04410 (LOX93_04410)	874552..875535	-	984	WP_243939156.1	tRNA-modifying protein YgfZ	-
LOX93_RS04415 (LOX93_04415)	875781..876047	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
LOX93_RS04420 (LOX93_04420)	876028..876435	+	408	WP_004144731.1	protein YgfX	Toxin
LOX93_RS04425 (LOX93_04425)	876442..876963	-	522	WP_002916308.1	flavodoxin FldB	-
LOX93_RS04430 (LOX93_04430)	877064..877960	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
LOX93_RS04435 (LOX93_04435)	877983..878696	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LOX93_RS04440 (LOX93_04440)	878702..880435	+	1734	WP_004151783.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15936.69 Da
• Isoelectric Point: 11.4778

>T224918 WP_004144731.1 NZ_CP087151:876028-876435 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 408 bp

>T224918 NZ_CP120622:c1298675-1297929 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT224918 WP_002916312.1 NZ_CP087151:875781-876047 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT224918 NZ_CP120622:c1297929-1297759 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A378E4P6

Antitoxin

• AlphaFold DB: A0A0H3GY41