Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 505239..505875 Replicon chromosome
Accession NC_009848
Organism Bacillus pumilus SAFR-032

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag BPUM_RS02380 Protein ID WP_003214169.1
Coordinates 505525..505875 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag BPUM_RS02375 Protein ID WP_012008995.1
Coordinates 505239..505520 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BPUM_RS02355 501180..501785 - 606 WP_012008991.1 rhomboid family intramembrane serine protease -
BPUM_RS02360 501880..502245 + 366 WP_012008992.1 holo-ACP synthase -
BPUM_RS02365 502406..503422 + 1017 WP_088004450.1 outer membrane lipoprotein carrier protein LolA -
BPUM_RS02370 503764..504942 + 1179 WP_012008994.1 alanine racemase -
BPUM_RS02375 505239..505520 + 282 WP_012008995.1 hypothetical protein Antitoxin
BPUM_RS02380 505525..505875 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BPUM_RS02385 505990..506820 + 831 WP_012008996.1 RsbT co-antagonist protein RsbRA -
BPUM_RS02390 506825..507193 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
BPUM_RS02395 507196..507597 + 402 WP_003214085.1 anti-sigma regulatory factor -
BPUM_RS02400 507608..508615 + 1008 WP_012008997.1 PP2C family protein-serine/threonine phosphatase -
BPUM_RS02405 508675..509004 + 330 WP_012008998.1 anti-sigma factor antagonist -
BPUM_RS02410 509001..509489 + 489 WP_012008999.1 anti-sigma B factor RsbW -
BPUM_RS02415 509455..510243 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
BPUM_RS02420 510243..510842 + 600 WP_012009001.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T22491 WP_003214169.1 NC_009848:505525-505875 [Bacillus pumilus SAFR-032]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T22491 NC_009848:505525-505875 [Bacillus pumilus SAFR-032]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGGTACGGCTTTGAACGAGATTCTGTCATTCTTTTAGAACAAATT
CGTACAATCGATAAACAAAGACTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT22491 WP_012008995.1 NC_009848:505239-505520 [Bacillus pumilus SAFR-032]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT22491 NC_009848:505239-505520 [Bacillus pumilus SAFR-032]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References