Detailed information of TA system TA224909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 84093..85003
• Replicon: plasmid unnamed1
• Accession: NZ_CP087147
• Organism: Klebsiella pneumoniae strain KP16

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LOX92_RS27495
• Protein ID: WP_004026354.1
• Coordinates: 84093..84563 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LOX92_RS27500
• Protein ID: WP_004026357.1
• Coordinates: 84560..85003 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX92_RS27455 (LOX92_27455)	79409..79723	+	315	WP_011251273.1	hypothetical protein	-
LOX92_RS27460 (LOX92_27460)	79732..80226	+	495	WP_011251274.1	hypothetical protein	-
LOX92_RS27465 (LOX92_27465)	80232..80672	+	441	WP_011251275.1	hypothetical protein	-
LOX92_RS27470 (LOX92_27470)	81097..82053	-	957	WP_011251280.1	DsbA family protein	-
LOX92_RS27475 (LOX92_27475)	82113..82454	-	342	WP_011251281.1	hypothetical protein	-
LOX92_RS27480 (LOX92_27480)	82468..82779	-	312	WP_011251282.1	hypothetical protein	-
LOX92_RS27485 (LOX92_27485)	82796..83245	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LOX92_RS27495 (LOX92_27495)	84093..84563	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LOX92_RS27500 (LOX92_27500)	84560..85003	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LOX92_RS27505 (LOX92_27505)	85104..85607	-	504	Protein_97	DUF4113 domain-containing protein	-
LOX92_RS27510 (LOX92_27510)	85813..86793	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
LOX92_RS27515 (LOX92_27515)	86859..88166	+	1308	Protein_99	FepA family TonB-dependent siderophore receptor	-
LOX92_RS27520 (LOX92_27520)	88228..88449	+	222	WP_004213615.1	hypothetical protein	-
LOX92_RS27525 (LOX92_27525)	88592..89494	-	903	WP_004213613.1	DMT family inner membrane transporter PEG344	-
LOX92_RS27530 (LOX92_27530)	89567..89968	-	402	WP_214588034.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..197415	197415
	-	inside	IScluster/Tn	-	iroN / rmpA	77812..97788	19976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T224909 WP_004026354.1 NZ_CP087147:c84563-84093 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T224909 NZ_CP120613:2936340-2936516 [Bacillus subtilis]
GTGCTTGAGAAAATGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT224909 WP_004026357.1 NZ_CP087147:c85003-84560 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT224909 NZ_CP120613:c2936557-2936457 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9