Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5395351..5395976 Replicon chromosome
Accession NZ_CP087146
Organism Klebsiella pneumoniae strain KP16

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LOX92_RS26655 Protein ID WP_002882817.1
Coordinates 5395351..5395734 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LOX92_RS26660 Protein ID WP_004150355.1
Coordinates 5395734..5395976 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX92_RS26640 (LOX92_26640) 5392717..5393619 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LOX92_RS26645 (LOX92_26645) 5393616..5394251 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LOX92_RS26650 (LOX92_26650) 5394248..5395177 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LOX92_RS26655 (LOX92_26655) 5395351..5395734 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LOX92_RS26660 (LOX92_26660) 5395734..5395976 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LOX92_RS26665 (LOX92_26665) 5396181..5397098 + 918 WP_002882812.1 alpha/beta hydrolase -
LOX92_RS26670 (LOX92_26670) 5397112..5398053 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LOX92_RS26675 (LOX92_26675) 5398098..5398535 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LOX92_RS26680 (LOX92_26680) 5398532..5399392 - 861 WP_002882807.1 virulence factor BrkB family protein -
LOX92_RS26685 (LOX92_26685) 5399386..5399985 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T224906 WP_002882817.1 NZ_CP087146:c5395734-5395351 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T224906 NZ_CP120613:c2478067-2477888 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT224906 WP_004150355.1 NZ_CP087146:c5395976-5395734 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT224906 NZ_CP120613:2477742-2477964 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References