Detailed information of TA system TA224889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 323576..324162
• Replicon: chromosome
• Accession: NZ_CP087146
• Organism: Klebsiella pneumoniae strain KP16

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LOX92_RS01505
• Protein ID: WP_002920800.1
• Coordinates: 323794..324162 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: LOX92_RS01500
• Protein ID: WP_002920802.1
• Coordinates: 323576..323797 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX92_RS01480 (LOX92_01480)	319733..320659	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LOX92_RS01485 (LOX92_01485)	320656..321933	+	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
LOX92_RS01490 (LOX92_01490)	321930..322697	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LOX92_RS01495 (LOX92_01495)	322699..323412	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LOX92_RS01500 (LOX92_01500)	323576..323797	+	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOX92_RS01505 (LOX92_01505)	323794..324162	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LOX92_RS01510 (LOX92_01510)	324435..325751	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LOX92_RS01515 (LOX92_01515)	325858..326745	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LOX92_RS01520 (LOX92_01520)	326742..327587	+	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LOX92_RS01525 (LOX92_01525)	327589..328659	+	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	318562..329396	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T224889 WP_002920800.1 NZ_CP087146:323794-324162 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T224889 NZ_CP120577:499868-500218 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT224889 WP_002920802.1 NZ_CP087146:323576-323797 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT224889 NZ_CP120577:499582-499863 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4