Detailed information of TA system TA224888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 88113..88693
• Replicon: plasmid unnamed3
• Accession: NZ_CP087145
• Organism: Klebsiella pneumoniae strain KP15

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LOX91_RS29500
• Protein ID: WP_071177730.1
• Coordinates: 88113..88427 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LOX91_RS29505
• Protein ID: WP_000093040.1
• Coordinates: 88415..88693 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX91_RS29475 (LOX91_29475)	84057..86021	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
LOX91_RS29480 (LOX91_29480)	86021..86752	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
LOX91_RS29485 (LOX91_29485)	86759..87289	+	531	WP_071177729.1	hypothetical protein	-
LOX91_RS29490 (LOX91_29490)	87316..87495	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LOX91_RS29495 (LOX91_29495)	87521..87949	-	429	WP_001140599.1	hypothetical protein	-
LOX91_RS29500 (LOX91_29500)	88113..88427	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOX91_RS29505 (LOX91_29505)	88415..88693	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LOX91_RS29510 (LOX91_29510)	88868..89233	-	366	WP_072354022.1	TonB family protein	-
LOX91_RS29515 (LOX91_29515)	89230..89601	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
LOX91_RS29520 (LOX91_29520)	89875..90120	-	246	WP_032440458.1	hypothetical protein	-
LOX91_RS29525 (LOX91_29525)	90765..92252	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
LOX91_RS29530 (LOX91_29530)	92330..92755	+	426	WP_045325066.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LOX91_RS29535 (LOX91_29535)	92755..93534	+	780	WP_108970993.1	Y-family DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / catA2 / tet(A) / blaLAP-2 / qnrS1 / dfrA14	-	1..99065	99065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T224888 WP_071177730.1 NZ_CP087145:c88427-88113 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T224888 NZ_CP120569:72534-72932 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT224888 WP_000093040.1 NZ_CP087145:c88693-88415 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT224888 NZ_CP120569:72307-72534 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1