Detailed information of TA system TA022488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 108437..108984
• Replicon: plasmid pAPEC-O1-ColBM
• Accession: NC_009837
• Organism: Escherichia coli APEC O1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: APECO1_RS26315
• Protein ID: WP_000969982.1
• Coordinates: 108703..108984 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: APECO1_RS26310
• Protein ID: WP_000079941.1
• Coordinates: 108437..108706 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO1_RS31670	103670..104656	-	987	Protein_114	IS4-like element IS421 family transposase	-
APECO1_RS31675	104657..105241	-	585	Protein_115	colicin V synthesis protein	-
APECO1_RS26290	105216..106490	-	1275	WP_000489607.1	HlyD family secretion protein	-
APECO1_RS26310	108437..108706	+	270	WP_000079941.1	hypothetical protein	Antitoxin
APECO1_RS26315	108703..108984	+	282	WP_000969982.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APECO1_RS26320	109030..109878	+	849	WP_001058005.1	3'-5' exonuclease	-
APECO1_RS26325	109995..110477	+	483	WP_001311056.1	hypothetical protein	-
APECO1_RS26330	110779..111126	+	348	WP_000142437.1	hypothetical protein	-
APECO1_RS26340	111685..112720	+	1036	Protein_123	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..174241	174241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10877.57 Da
• Isoelectric Point: 7.2749

>T22488 WP_000969982.1 NC_009837:108703-108984 [Escherichia coli APEC O1]
MMEIFWTMLASQDRKCIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T22488 NC_009837:108703-108984 [Escherichia coli APEC O1]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGTGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT22488 WP_000079941.1 NC_009837:108437-108706 [Escherichia coli APEC O1]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT22488 NC_009837:108437-108706 [Escherichia coli APEC O1]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5