Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5398320..5398945 Replicon chromosome
Accession NZ_CP087142
Organism Klebsiella pneumoniae strain KP15

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LOX91_RS26675 Protein ID WP_002882817.1
Coordinates 5398320..5398703 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LOX91_RS26680 Protein ID WP_004150355.1
Coordinates 5398703..5398945 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX91_RS26660 (LOX91_26660) 5395686..5396588 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LOX91_RS26665 (LOX91_26665) 5396585..5397220 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LOX91_RS26670 (LOX91_26670) 5397217..5398146 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LOX91_RS26675 (LOX91_26675) 5398320..5398703 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LOX91_RS26680 (LOX91_26680) 5398703..5398945 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LOX91_RS26685 (LOX91_26685) 5399150..5400067 + 918 WP_002882812.1 alpha/beta hydrolase -
LOX91_RS26690 (LOX91_26690) 5400081..5401022 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LOX91_RS26695 (LOX91_26695) 5401067..5401504 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LOX91_RS26700 (LOX91_26700) 5401501..5402361 - 861 WP_002882807.1 virulence factor BrkB family protein -
LOX91_RS26705 (LOX91_26705) 5402355..5402954 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T224879 WP_002882817.1 NZ_CP087142:c5398703-5398320 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T224879 NZ_CP120568:c2543528-2543352 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT224879 WP_004150355.1 NZ_CP087142:c5398945-5398703 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT224879 NZ_CP120568:c2543306-2542890 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References