Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4813592..4814402 Replicon chromosome
Accession NZ_CP087142
Organism Klebsiella pneumoniae strain KP15

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LOX91_RS23880 Protein ID WP_002887280.1
Coordinates 4813592..4814125 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LOX91_RS23885 Protein ID WP_002887278.1
Coordinates 4814136..4814402 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX91_RS23875 (LOX91_23875) 4812423..4813544 + 1122 WP_002887282.1 cupin domain-containing protein -
LOX91_RS23880 (LOX91_23880) 4813592..4814125 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LOX91_RS23885 (LOX91_23885) 4814136..4814402 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LOX91_RS23890 (LOX91_23890) 4814505..4815938 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
LOX91_RS23895 (LOX91_23895) 4815928..4816611 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LOX91_RS23900 (LOX91_23900) 4816783..4818168 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LOX91_RS23905 (LOX91_23905) 4818186..4818530 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T224876 WP_002887280.1 NZ_CP087142:c4814125-4813592 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T224876 NZ_CP120568:2098093-2098196 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT224876 WP_002887278.1 NZ_CP087142:c4814402-4814136 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT224876 NZ_CP120568:c2098234-2097963 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References