Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4198397..4199016 Replicon chromosome
Accession NZ_CP087142
Organism Klebsiella pneumoniae strain KP15

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LOX91_RS20925 Protein ID WP_002892050.1
Coordinates 4198798..4199016 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LOX91_RS20920 Protein ID WP_002892066.1
Coordinates 4198397..4198771 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX91_RS20910 (LOX91_20910) 4193549..4194742 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LOX91_RS20915 (LOX91_20915) 4194765..4197911 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LOX91_RS20920 (LOX91_20920) 4198397..4198771 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LOX91_RS20925 (LOX91_20925) 4198798..4199016 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LOX91_RS20930 (LOX91_20930) 4199175..4199741 + 567 WP_002892030.1 maltose O-acetyltransferase -
LOX91_RS20935 (LOX91_20935) 4199713..4199853 - 141 WP_004147370.1 hypothetical protein -
LOX91_RS20940 (LOX91_20940) 4199874..4200344 + 471 WP_002892026.1 YlaC family protein -
LOX91_RS20945 (LOX91_20945) 4200319..4201770 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LOX91_RS20950 (LOX91_20950) 4201871..4202569 + 699 WP_002892021.1 GNAT family protein -
LOX91_RS20955 (LOX91_20955) 4202566..4202706 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LOX91_RS20960 (LOX91_20960) 4202706..4202969 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224875 WP_002892050.1 NZ_CP087142:4198798-4199016 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T224875 NZ_CP120568:1073109-1073444 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT224875 WP_002892066.1 NZ_CP087142:4198397-4198771 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT224875 NZ_CP120568:1072861-1073109 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References